TAILIEUCHUNG - In vitro growth, antrum formation and Metaphase II development of preantral follicles in sheep with Estradiol-17β supplementation at different time points

The present study was conducted to investigate the influence of sequential addition of estradiol-17β on in vitro development of preantral follicles (PFs’) in sheep. Preantral follicles isolated from the ovarian cortical slices using micro dissection method were cultured for six days in Bicarbonate buffered Tissue culture medium 199B (TCM 199B) or in a standard culture medium supplemented with Estradiol-17β (5 ng/ml) at different points during the culture period. COCs isolated from the follicles at the end of six day culture in different treatments were subjected to in vitro maturation for additional 24h. Supplementation of estradiol-17β during first two days of the culture supported better average increase in diameter and proportion of PFs' exhibiting antrum formation at the end of six day culture. Further the oocytes in COCs isolated at the end of culture in these treatments and subsequently subjected to in vitro maturation (IVM) for 24hr developed at a higher frequency to MII (metaphase II) stage. Supplementation of Estradiol-17β to TCM 199B culture medium in early stages followed by standard medium alone in later stages supports better development of PFs' in vitro. Following supplementation with estradiol-17β for the first two days culture of PFs' in standard medium appears to be advantageous for the development of preantral follicles in vitro. | In vitro growth, antrum formation and Metaphase II development of preantral follicles in sheep with Estradiol-17β supplementation at different time points

• Sinh học
• In vitro growth
• Antrum formation and Metaphase II
• Development of preantral follicles
• Estradiol 17β supplementation
• Different time points
• Hormone Growth
• Insulin Growth Factor 1
• Tăng trưởng xương
• Nghiên cứu In vitro
• Nuôi cấy mô xương
• Nguyên bào xương
• Tissue culture
• In vitro shoot induction
• Growing media
• Growth regulators
• Impact of growing media
• In vitro culture of aloe vera
• Carica papaya
• Auxins and cytokinins
• Growth regulators on in vitro cloning
• In vitro cloning
• Shoot tip and inflorescences
• In vitro
• In vitro mutagenesis
• In vivo growth characteristics of strawberry
• Application duration on spread of the plant
• Explants of strawberry
• Different plant growth
• Different plant growth regulators
• In vitro callus induction
• Various concentrations of NAA
• Biocontrol agent
• In vitro effect of culture filtrates
• Culture filtrates of Chaetomium Globosum
• Chaetomium Globosum on growth
• Growth of soil borne pathogens
• Pseudomonas fluorescens
• Rhizoctonia solani
• Dual culture
• In vitro and in soil
• Rhizoctonia solani in vitro and in soil
• Mycelial growth
• Activated charcoal
• Potato varieties
• Potato (Solanum tuberosum L )
• Activated charcoal on in vitro growth
• Sheath blight
• Different herbicides on the mycelial growth
• Mycelial growth of rhizoctonia solani
• Rhizoctonia solani in vitro
• Studies on Isolation
• Characterization and in vitro screening
• In vitro screening of plant
• Promoting rhizobacteria
• Rhizospheric soil of chrysanthemum
• Dendranthema grandiflora Tzvelev
• Gladiolus hybrida
• In vitro regeneration
• Gladiolus Gladiolus hybrida
• In vitro multiple shoots production
• Cormel shoot buds
• Alternaria tenuissima
• Leaf blight of kodo millet
• In vitro efficacy of fungicides
• Employing poison food technique
• In vitro micro propagation regeneration
• Chickpea plant
• Regeneration of chickpea plant
• In vitro micro propagation
• plant growth regulators NAA
• Serapias vomeracea
• Synthetic seed
• In vitro seed germination
• Plantlet growth
• Icropropagation techniques
• Sclerotium rolfsii
• collar rot of Indian bean
• Sclerotium rolfsii in vitro on collar rot
• Light and pH on the growth
• Several crops
• Fungal pathogen
• pH levels
• In vitro condition
• Early blight of tomato
• Tomato (Solanum lycopersicum L
• Exploring the role of glucose
• Optimizing in vitro growth
• Bacterial isolates
• Aluminium stressed conditions
• Bacterial endophytes
• Plant growth promotion
• Vitro condition
• Evaluation of bacterial endophytes
• Indirectly by several mechanisms
• Micro organism
• Antagonistic and synergistic
• Neem (Azadirachta indica)
• Lantana (Lantana camara) leaf extract
• Growth of Fusarium solani
• In vitro conditions
• Soybean [Glycine max (L ) Merrill]
• Screening of Bradyrhizobial isolates
• Plant growth promoting properties
• Organic or inorganic ions
• Fusarium oxysporum f sp udum
• Sporulation and temperature
• pH on growth
• sporulation of Fusarium oxysporum
• Colletotrichum graminicola
• Culture media
• Anthracnose of sorghum
• In vitro evaluation
• Various culture media
• Growth of Colletotrichum graminicola
• RAPD markers
• gladiolus (Gladiolus hybrida L )
• Multi walled carbon nano tubes
• Functionalized carbon nano tubes
• Malbhog banana
• Plant growth regulators