TAILIEUCHUNG - Chemical composition and antibacterial activity of fractions from Bridelia micrantha stem bark Methanol extract

Bridelia micrantha commonly known as coastal golden leaf is a member of the family Phyllanthaceae. In preliminary studies, nine fractions, named F1- F9, were obtained by fractionating the crude methanol extract of the stem bark of Bridelia micrantha using column chromatographic techniques. The F6 fraction was found to be the most active when tested for the antibacterial activity. This study is thus aimed at investigating the effect of fractionation on antibacterial activity of F6 fraction. The F6 fraction was fractionated by adsorption chromatography on silica gel into eight sub-fractions designated F’1- F’8. A product was isolated from the dichloromethane/ methanol (10%) fraction and the structure was determined on the basis of spectroscopic data. The antibacterial activity of the F6 fraction, sub-fractions and the product was evaluated by broth microdilution method against two reference strains and eighteen clinical bacterial strains. The chemical analysis of F6 and three sub-fractions F’3, F’4 and F’5 was done using HPLC-MS. The fraction F6exhibited strong activity on all the tested bacteria with MIC values of 128 µg/ml on nine strains, including Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and Salmonella sub-fractions F’4 and F’5 exhibited the best activities on all the tested bacteria with MIC values of 32 to 256 µg/ml. The chemical analysis by HPLC-MS of F6, F’3, F’4 and F’5 revealed the presence of almost 180 identified compounds from various classes of secondary metabolites including alkaloids, flavonoids, steroids and terpenoids. The product obtained although inactive was elucidated as Daucosterol. | Chemical composition and antibacterial activity of fractions from Bridelia micrantha stem bark Methanol extract

• Hoá học
• Bridelia micrantha
• Methanol extract
• Antibacterial activity
• Bridelia micrantha stem bark Methanol extract
• Chemical composition
• Physical character
• Different sorghum genotypes
• Grain physico chemical composition
• Variability in grain physico chemical composition
• Chemical Composition of the essential oil
• The essential oil of Miliusa Baillonii Pierre
• Content of essential oils
• To fresh materials
• Essential oils of Annona Aquamosa L
• Chemical composition of essential oils
• Annona Reticulata L
• Sắc ký khí phổi phổ liên hợp
• Summary of Chemical engineering Doctoral Thesis
• Chemical engineering Doctoral Thesis
• Docynia indica
• Make valuable products
• Develop extraction technology process
• Apple pomace
• Fungal treated straw
• Ensiled apple pomace paddy straw
• Evaluation of chemical composition
• Lecture Meat science and Meat processing
• Meat science and Meat processing
• Chemical composition of meat
• Structure of meat
• Composition of triglyceride
• Lepidium sativum
• Proximate composition
• Mineral composition
• Garden cress (Lepidium sativum L )
• Luận án Tiến sĩ
• Sheic species
• Large leafed Tuzhou
• Summary of Chemical doctoral thesis
• Biological activity
• Luận án Tiến sĩ Hóa học
• Chemical engineering
• Ectomycorrhizal mushroom
• Tricholoma anatolicum
• Total carbohydrate content
• Genomic status
• Chemical and mineral composition
• Human nutrition
• Genomic groups grown in India
• Carrot germplasms
• Physical parameter
• Physicochemical composition
• Newer carrot germplasms
• Physico chemical composition
• Chemical Equilibrium
• Engineering Biotechnology
• Analytical Chemistry
• chemical analysis
• Zeolite (clinoptilolite) on chemical parameters of rabbit meat
• Chemical parameters of rabbit meat
• The rabbit's meat chemical composition
• Individual amino acid
• Musculus Longissimus dorsi
• Environmental advances
• Bayer effluent Gardanne alumina plant
• Mineralogical composition
• The Mediterranean Sea
• Bayer liquor
• Bayer residue
• Litsea glutinosa
• natural sciences
• science and technology
• scientific reports
• research projects
• scientific research
• Công nghệ hóa
• Angelica sinensiss
• Containing compound
• Hóa hữu cơ
• Hợp chất nito
• Fodder sorghum
• Harvest period
• Sudan grass
• High nutritional value
• Journal of law and criminal justice
• Influence of nitrogen fertilization
• The chemical composition of Paspalum plicatulum
• Differents phenological stages
• The central plateau of Fouta Djallon
• Broiler chicken
• Breast muscle
• Breast muscle in broiler chicken
• Higher dietary l arginine supplementation
• Mugil cephalus
• Effect of herbal liver tonic
• Bio chemical composition
• Grey mullet Mugil cephalus
• Mugil cephalus (Linnaeus) fry
• Chemical composition f Hypochaeris radicata L
• Inhibitory effects of Hypochaeris radicata L extracts
• Hypochaeris radicata L extracts on seed germination
• Growth of Echinochloa crus galli L
• Cinnamon oil
• Anatomical features of roots
• Antibacterial activities of cinnamon oil
• Cinnamon oil (Cinnamomum cassia (L ) J PRESL)
• Bypass fat
• Crossbred cows
• Milk yield
• Milk composition
• Chemical parameters of ghee