TAILIEUCHUNG - The effect of in vitro competition on shoot regeneration from hypocotyl explants of Linum usitatissimum

This study was carried out to investigate the relationship between in vitro competition and stress, and their effects on tissue culture response of Linum usitatissimum L. hypocotyl explants. Competition among explants was achieved by varying the spacing among the explants cultured. | M. YILDIZ, Ç. SAĞLIK, C. TELCİ, E. G. ERKILIÇ Research Article Turk J Bot 35 (2011) 211-218 © TÜBİTAK doi: The effect of in vitro competition on shoot regeneration from hypocotyl explants of Linum usitatissimum Mustafa YILDIZ1,*, Çağlayan SAĞLIK2, Cansu TELCİ1, Emine Gülden ERKILIÇ2 1 University of Ankara, Faculty of Agriculture, Department of Field Crops, 06110 Dışkapı, Ankara - TURKEY 2 University of Ankara, Graduate School of Biotechnology, Department of Basic Biotechnology, 06500 Beşevler, Ankara - TURKEY Received: Accepted: Abstract: This study was carried out to investigate the relationship between in vitro competition and stress, and their effects on tissue culture response of Linum usitatissimum L. hypocotyl explants. Competition among explants was achieved by varying the spacing among the explants cultured. Four different culture spacing distances were used: , , , and cm. Six weeks after culture initiation, hypocotyl fresh and dry weights, shoot regeneration percentage, shoot number per hypocotyl, regenerated shoot length, total shoot number per petri dish, and total chlorophyll content were recorded. The results showed that encouraging competition among explants by decreasing spacing among them from cm to cm increased shoot number per hypocotyl, regenerated shoot length, and total shoot number per petri dish in both cultivars. When explants were cultured at cm spacing, significant stress-initiated decreases were observed in all parameters examined. This study showed that the success of tissue culture studies for related genotype could be increased not only by determination of correct concentrations and combinations of auxins and cytokinins in growth medium but also by evaluating competition among explants cultured. Key words: Linum usitatissimum, hypocotyl, in vitro competition, shoot regeneration In vitro rekabetin Linum usitatissimum hipokotil eksplantlarından sürgün .

• Nông nghiệp
• Linum usitatissimum
• hypocotyl explants
• In vitro competition
• Shoot regeneration
• Explants cultured
• Linseed (Linum usitatissimum L )
• Harvest index
• Yield and yield components
• Germplasm Estimate heterosis
• Linum usitatissimum L
• Path analysis
• Principal component analysis
• Linseed genotypes (Linum usitatissimum L )
• Various selection parameters
• Genetic basis of variation
• Linseed Linum usitatissimum L
• Morpho physiological characteristics
• Self pollinating crop
• Gene pool of Linum usitatissimum
• Anther culture potential
• Effects of genotypes
• Cultivar Blue Chip gave
• Agar solidified
• Fiber cell thickness
• Mineral salts and vitamins
• Tissue culture
• Vitamin rich MS medium
• Flax Linum usitatissimum L
• Injured area
• Callus based shoot regeneration
• Agrobacterium inoculation
• Hypocotyl explants of flax plants
• In vitro shoot regeneration
• Pretreatment application
• 6 benzylaminopurine
• L 1 naphthalene acetic acid
• Genotypic correlation coefficient
• Phenotypic correlation
• Aerial biomass
• Linseed (Linum usitatissimum L
• Combining ability
• Seed yield and linseed
• Linseed (Linum usitatissimum L)
• Phonological traits in linseed
• Estimation of heterosis
• Half diallel analysis
• Bud fly infestation
• Trichome length
• Bud damage in linseed
• Influence of trichome length
• D2 statistics
• Intra cluster
• Diversity analysis in linseed
• Diversity analysis
• Inbreeding depression
• Heterosis and inbreeding depression
• Exploitation of heterosis
• High degree of heterosis
• Acid value
• Fatty acids
• Colour value
• Seed parameters in linseed
• F2 generation
• Correlation coefficient
• Direct effect
• Indirect effect and path coefficient analysis
• Path coefficient analysis
• Flax type linseed genotypes
• Assessment of variability
• Hybridization programme
• Gene conferring resistance to rust
• Rust (Melampsora lini)
• Gene conferring resistance
• Melamspora lini
• Fusarium wilt
• Suitable sowing dates
• Varieties for the management of linseed
• Wilt under wilt sick field conditionincited
• BMC Plant Biology
• Saline alkaline stress
• Deep sequencing
• Abiotic stress
• Genetic diversity
• D 2 statistics
• Clustering pattern
• Genetic diversity in linseed
• Quizalofop ethyl
• Weed management
• Cultural method of weed control on growth
• Yield and quality of linseed
• Basal diameter
• Suitable nutrient management
• Dual purpose flax (Linum usitatissimum L )
• New alluvial zone
• Dual purpose flax crop
• Line x tester
• Heterosis for seed yield
• Attributing traits in linseed
• Line × tester mating design
• Physicochemical and chemical characteristics
• Linseed equivalent yield
• Production efficiency
• Chemical characteristics
• Rainfed condition
• Genetic variability
• Induced mutation
• EMS induced mutagenesis
• Isolation of high yielding mutants