TAILIEUCHUNG - Anther culture potential of linseed (Linum usitatissimum L.): Effects of genotypes and pretreatment on callus formation and differentiation

This study is concerned with the production of haploids of linseed. Anthers of eight genotypes were cultured both on solid and in liquid medium. It was found that callus induction rate was low in both media and also the cultivars significantly affected callus induction rate both on solid and in liquid medium. | Tr. J. of Agriculture and Forestry 22 (1998) 553-560 © TÜBİTAK Anther Culture Potential of Linseed (Linum usitatissimum L.): Effects of Genotypes and Pretreatment on Callus Formation and Differentiation Orhan KURT University of Ondokuz Mayıs, Faculty of Agriculture, Department of Agronomy, Samsun-TURKEY Gareth. M. EVANS University of Wales, Department of Agricultural Sciences, Aberystwyth-UK Received: Abstract: This study is concerned with the production of haploids of linseed. Anthers of eight genotypes were cultured both on solid and in liquid medium. It was found that callus induction rate was low in both media and also the cultivars significantly affected callus induction rate both on solid and in liquid medium. The cultivar Blue-Chip gave, on average, the maximum () callus induction rate on solid medium and the cultivar Antares gave, on average, the maximum () callus induction rate in liquid medium. The cultivar Norman produced callus on solid medium but did not produce any callus in liquid medium. On the other hand, the cultivar McGregor did not produce any callus in either solid or liquid medium. Pre-treatment of flower buds with cold significantly reduced callus induction rate by about four fold compared with the no pre-treatment on solid medium but increased (not statistically significant) callus induction rate by in liquid medium. Although some callus induction was achieved in both on solid and in liquid media, no differentiation was observed when calli were transferred to regeneration medium. Keten (Linum usitatissimum L.)’de Anter Kültürünün Potansiyeli: Kallus Oluşumu ve Farklılaşmasına Genotip ve Ön Muamelenin Etkisi Özet: Bu araştırma haploid keten bitkilerinin elde edilmesi ile ilgili o lup, 8 keten çeşitinin anterleri katı ve sıvı kültür ortamında kültüre alınmıştır. Araştırma sonucu her iki ortamda da kallus teşekkül oranının düşük olduğu belirlenmiştir. Ayrıca, kallus teşekkül oranı bakımından her iki ortamda da .

• Nông nghiệp
• Linseed Linum usitatissimum L
• Anther culture potential
• Effects of genotypes
• Cultivar Blue Chip gave
• Agar solidified
• Linseed (Linum usitatissimum L )
• Harvest index
• Yield and yield components
• Germplasm Estimate heterosis
• Linum usitatissimum L
• Path analysis
• Principal component analysis
• Linseed genotypes (Linum usitatissimum L )
• Various selection parameters
• Combining ability
• Seed yield and linseed
• Linseed (Linum usitatissimum L)
• Phonological traits in linseed
• Estimation of heterosis
• Half diallel analysis
• Genetic basis of variation
• Morpho physiological characteristics
• Self pollinating crop
• Gene pool of Linum usitatissimum
• D2 statistics
• Intra cluster
• Linseed (Linum usitatissimum L
• Diversity analysis in linseed
• Diversity analysis
• Flax type linseed genotypes
• Assessment of variability
• Hybridization programme
• Fusarium wilt
• Suitable sowing dates
• Varieties for the management of linseed
• Wilt under wilt sick field conditionincited
• Line x tester
• Heterosis for seed yield
• Attributing traits in linseed
• Line × tester mating design
• Physicochemical and chemical characteristics
• Linseed equivalent yield
• Production efficiency
• Chemical characteristics
• Rainfed condition
• Genetic diversity
• D 2 statistics
• Clustering pattern
• Genetic diversity in linseed
• Quizalofop ethyl
• Weed management
• Cultural method of weed control on growth
• Yield and quality of linseed
• Genotypic correlation coefficient
• Phenotypic correlation
• Aerial biomass
• Bud fly infestation
• Trichome length
• Bud damage in linseed
• Influence of trichome length
• Inbreeding depression
• Heterosis and inbreeding depression
• Exploitation of heterosis
• High degree of heterosis
• Acid value
• Fatty acids
• Colour value
• Seed parameters in linseed
• F2 generation
• Correlation coefficient
• Direct effect
• Indirect effect and path coefficient analysis
• Path coefficient analysis
• Gene conferring resistance to rust
• Rust (Melampsora lini)
• Gene conferring resistance
• Melamspora lini
• BMC Genomics
• Repeated drought
• Transcription factors
• Oil flax
• Linseed coordinates
• Genetic variability
• Induced mutation
• Linum usitatissimum
• EMS induced mutagenesis
• Isolation of high yielding mutants
• Yellow seeded linseed
• Association correlation analysis
• Seed yield contributing traits
• Yellow seeded genotypes of linseed
• Yellow seeded genotypes
• Natural farming
• Nutrient management
• Oil and linseed
• Soil properties in linseed
• NPK uptake
• Genetic advance
• Path analysis for yield in linseed
• Yield in linseed
• Genetic parameters of variability
• Economics of linseed
• Yield attributes and economics
• Integrated weed management
• Integrated weed management on growth
• Date of sowing
• Linseed varieties
• Yield attributes and dryland
• Dryland condition
• Sowing on yield attributes