TAILIEUCHUNG - Increasing the injured area on hypocotyl explants of flax (Linum usitatissimum L.) leads to high frequency callus based shoot regeneration

In this study, it was aimed to increase the frequency of shoots, which would be nominated to be transgenic after Agrobacterium inoculation, through expanding the wounding area by peeling the epidermis layer of hypocotyl explants of flax plants. | Turk J Biol 26 (2002) 95-98 © TÜB‹TAK Increasing the Injured Area on Hypocotyl Explants of Flax (Linum usitatissimum L.) Leads to High Frequency Callus-Based Shoot Regeneration Mustafa YILDIZ, Celâl ER . Faculty of Agriculture, Department of Field Crops, 06110 Ankara-TURKEY Received: Abstract: In this study, it was aimed to increase the frequency of shoots, which would be nominated to be transgenic after Agrobacterium inoculation, through expanding the wounding area by peeling the epidermis layer of hypocotyl explants of flax plants. Callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish were recorded as g, 40%, and , respectively in non-peeled explants. On the other hand, when the epidermis layer was peeled, callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish increased to g, , and , respectively. Key Words: Flax, injured area, callus-based shoot regeneration Keten (Linum usitatissimum L.) Hipokotil Eksplantlar›nda Yaralanan Yüzeyin Art›r›lmas› Kallustan Geliflen Sürgün Frekans›n› Yükseltmektedir Özet: Bu araflt›rmada, keten bitkisinin hipokotil eksplantlar›nda, epidermis tabakas› soyularak yaralanan yüzeyin geniflletilmesi ile Agrobacterium inokulasyonundan sonra transgenik olmaya aday sürgün frekans›n›n art›r›lmas› amaçlanm›flt›r. Soyulmam›fl hipokotil eksplantlar›nda kallus a¤›rl›¤›, sürgün oluflturan hipokotil yüzdesi, eksplant bafl›na sürgün say›s› ve petrideki sürgün say›s› s›ras›yla g, %40, adet ve adet olarak belirlenmifltir. Di¤er taraftan, epidermis tabakas› soyuldu¤unda, kallus a¤›rl›¤›, sürgün oluflturan hipokotil yüzdesi, eksplant bafl›na sürgün say›s› ve petrideki sürgün say›s› s›ras›yla g, %, adet ve adete yükselmifltir. Anahtar Sözcükler: Keten, yaralanan yüzey, kallus tabanl› sürgün rejenerasyonu Introduction Several genes that

• Sinh học
• Flax Linum usitatissimum L
• Injured area
• Callus based shoot regeneration
• Agrobacterium inoculation
• Hypocotyl explants of flax plants
• Flax type linseed genotypes
• Linum usitatissimum L
• Assessment of variability
• Hybridization programme
• Basal diameter
• Suitable nutrient management
• Dual purpose flax (Linum usitatissimum L )
• New alluvial zone
• Dual purpose flax crop
• Alternaria blight
• Microalgae against alternaria blight of flax
• Blight of flax
• Fiber cell thickness
• Mineral salts and vitamins
• Tissue culture
• Vitamin rich MS medium
• BMC Genomics
• Transcription factor
• Phylogenetic analysis
• Expression patterns
• Bioinformatics based predictions
• Repeated drought
• Transcription factors
• Oil flax
• Linseed coordinates
• In vitro shoot regeneration
• Linum usitatissimum
• Pretreatment application
• 6 benzylaminopurine
• L 1 naphthalene acetic acid
• BMC Plant Biology
• Saline alkaline stress
• Deep sequencing
• Abiotic stress
• Explant sources
• Adventitious shoot regeneration
• Hypocotyl explants
• Omega flax cultivars
• Per petri dish
• Photoperiod sensitivity
• Canadian flax cultivars
• 5 azacytidine treated
• Plant Biology
• LEA gene identification
• Functional analysis
• Seed development
• Fatty acid
• Linseed flax
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• Germination time
• Predicted model
• Seed quality
• Cowpea seeds
• Describe plant growth
• Linkage map
• Plant height
• Technical length
• Quantitative trait locus
• ABC transporter
• Gene duplication
• Expression profiling
• HMA gene families
• Genetic diversity
• Linkage disequilibrium
• Germplasm collection
• Diverse genotypes
• Sustainable breeding program requires