TAILIEUCHUNG - The effect of drying and submersion pretreatment on adventitious shoot regeneration from hypocotyl explants of flax (Linum usitatissimum L.)

Hypocotyl explants of 3 flax cultivars were cultured for adventitious shoot regeneration in 3 different ways. Two pretreatment applications were compared with the routinely applied conventional regeneration protocol of culturing explants directly on Murashige and Skoog (MS) medium containing 1 mg L-1 6-benzylaminopurine (BAP) and mg L-1 naphthalene acetic acid (NAA). | Research Article Turk J Bot 34 (2010) 323-328 © TÜBİTAK doi: The effect of drying and submersion pretreatment on adventitious shoot regeneration from hypocotyl explants of flax (Linum usitatissimum L.) Mustafa YILDIZ*, Sebahattin ÖZCAN, Cansu TELCİ, Sibel DAY, Hande ÖZAT Ankara University, Faculty of Agriculture, Department of Field Crops, 06110 Dışkapı, Ankara - TURKEY Received: Accepted: Abstract: Hypocotyl explants of 3 flax cultivars were cultured for adventitious shoot regeneration in 3 different ways. Two pretreatment applications were compared with the routinely applied conventional regeneration protocol of culturing -1 explants directly on Murashige and Skoog (MS) medium containing 1 mg L 6-benzylaminopurine (BAP) and mg -1 L naphthalene acetic acid (NAA). In the first pretreatment application, explants kept in a sterile cabin under an air flow for 30 min were immersed in MS solution containing 1 mg L-1 BAP and mg L-1 NAA for 15 min; then pretreated explants were cultured on MS medium without any growth regulators (MS0). In the second pretreatment application, -1 explants were kept in a sterile cabin under air flow for 30 min and then immersed in MS solution containing 1 mg L -1 BAP and mg L NAA for 15 min. The pretreated explants were then transferred to a culture medium enriched with 1 mg L-1 BAP and mg L-1 NAA. Fresh and dry weights of hypocotyl explants, shoot regeneration percentage, shoot number per hypocotyl, shoot length, and total chlorophyll content were recorded. From the results, it could be seen that -1 treating explants before culture initiation for regeneration with a liquid MS medium containing 1 mg L BAP and -1 mg L NAA for 15 min after keeping hypocotyls under an air flow in a sterile cabin for 30 min gave rise to the highest scores for tissue culture response. Key words: In vitro shoot regeneration, Linum usitatissimum, pretreatment application Keten bitkisi (Linum .

• Nông nghiệp
• In vitro shoot regeneration
• Linum usitatissimum
• Pretreatment application
• 6 benzylaminopurine
• L 1 naphthalene acetic acid
• Multiple shoot regeneration
• Plumular apices of chickpea
• In vitro
• Shoot regeneration
• Genetic transformation
• Anthemis xylopoda
• Endemic plant
• Adventitious shoot regeneration
• In vitro flowering
• Indole3 acetic acid
• hypocotyl explants
• In vitro competition
• Explants cultured
• Shoot proliferation
• Sliced segment section
• Thanks giving cactus
• Gladiolus hybrida
• In vitro regeneration
• Growth regulators
• Gladiolus Gladiolus hybrida
• In vitro multiple shoots production
• Cormel shoot buds
• Somatic embryogenesis
• Simultaneous shoot
• Fast regeneration
• Root induction
• Sugarcane Saccharum officinarum L
• Aquatic plant
• Apical shoot
• Hemianthus callitrichoides
• Plant growth regulators
• Gibberellic acid
• BMC Plant Biology
• In vitro plant regeneration
• Daylilies tissue culture
• Shoot organogenesis
• Nutritional value
• Direct organonesis
• In vitro protocol for direct organogenesis
• In vitro protocol
• Mass multiplication
• Direct organogenesis
• Sliced mature embryo
• Chowghat oragne dwarf
• In vitro culture
• Plant Tissue
• Culture Media
• Emerging Economy
• Abiotic Stress Conditions
• Tissue Culture
• Verbena officinalis
• Stem internode explant
• Including antimicrobial
• Biological activities
• Osmotic treatment
• Cotton shoot regeneration
• Local cotton cultivar
• Cotton Gossypium hirsutum L
• Vigna unguiculata
• Agricultural constraints
• Biotechnological methods
• Indole 3 butyric acid
• In vitro regenerated plants
• Woody plant medium
• Shoot bud
• Santalum album
• Exogenous auxin
• Tertiary butyl alcohol