TAILIEUCHUNG - Multiple shoot regeneration of plumular apices of chickpea

Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world. Development of new repeatable and reliable tissue culture protocols is very important for improvement of breeding and genetic transformation studies. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2013) 37: 33-39 © TÜBİTAK doi: Multiple shoot regeneration of plumular apices of chickpea 1, 2 2 2 Muhammad AASIM *, Sibel DAY , Fereshteh REZAEI , Mortaza HAJYZADEH Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Yunus Emre Campus, 70200 Karaman, Turkey 2 Department of Field Crops, Faculty of Agriculture, Ankara University, 06110, Dışkapı, Ankara, Turkey 1 Received: Accepted: Published Online: Printed: Abstract: Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world. Development of new repeatable and reliable tissue culture protocols is very important for improvement of breeding and genetic transformation studies. It is a highly recalcitrant plant and there is an urgent need to develop a regeneration protocol that can ensure easy multiple and qualitative superior shoots that could be rooted and yield fertile plants. Unconditioned plumular apices and plumular apices preconditioned with 10 mg L–1 benzylaminopurine (BA) for 10 days were cultured on Murashige and Skoog (MS) medium supplemented with – mg L–1 BA with or without mg L–1 naphthalene acetic acid (NAA). Comparing the 2 culture conditions, the preconditioned explants had 2-fold to 5-fold more regeneration and shoots per explant compared to the unconditioned plumular apices. The presence of NAA in the culture medium positively increased the number of shoots per preconditioned explant at the lower concentrations of BA. In contrast, NAA inhibited the number of shoots per explants and the mean shoot length of the unconditioned explants at all concentrations of BA. An increased sucrose concentration of 45 and 60 g L–1 (R1) with mg L–1 indole-3 butyric acid resulted in 50% rooting with multiple and earlier hardening of shoots. .

• Nông nghiệp
• Multiple shoot regeneration
• Plumular apices of chickpea
• In vitro
• Shoot regeneration
• Genetic transformation
• Multiple shoot
• Physalis minima L
• Fruit and medicinal plant
• Shoot apical culture
• Cytokinin on multiple shoot regeneration
• Maize (Zea mays)
• Split nodes
• Multiple shoots
• Multiple shoot induction
• Direct regeneration
• Callus induction
• Indian wheat
• Mature embryos
• Rapid regeneration of Indian wheat cultivars
• Gladiolus hybrida
• In vitro regeneration
• Growth regulators
• Gladiolus Gladiolus hybrida
• In vitro multiple shoots production
• Cormel shoot buds
• Genetic transformation protocol
• Early flowering cryptochrome gene
• Taif rose plant
• The Taif Rose genotype were
• GUS gene
• Pigeon pea
• Cajanus cajan L Millsp
• In planta method of genetic transformation
• Squirting cucumber
• Agrobacterium tumefaciens
• Mediated transformation of plants
• PAoPR1 GUS INT plasmids
• pruriens
• Yeast extract peptone agar
• Nontransgenic plant DNA
• Polymerase chain reaction
• BMC Plant Biology
• Genotyping by sequencing
• Genetic map
• Win gene
• Homologous transformation sterility 1
• Agrobacterium tumefaciens transformation
• Gene editing
• Agrobacterium mediated genetic transformation
• Biolistic transformation
• health
• care
• primary
• report
• María Alejandra Alvarez
• Aromatic indica rices
• Immature embryos
• Aromatic Indica rices mediated
• coli
• Genetic modification
• gdhA gene
• Tobacco plants
• Back gram
• Black gram (Vigna mungo L )
• Cultivar VBN 3
• Agrobacterium mediated
• Planta genetic transformation
• Solanum trilobatum L
• Direct shoot organogenesis
• Histochemical gus analysis
• BMC Biotechnology
• Co transformation
• Gaussia princeps luciferase gene
• Genetic engineering
• Green algae
• Heterologous expression
• Reporter genes
• BMC Genetics
• Insect genetic modification
• Transposon mediated transformation
• Drosophila hobo element
• Bactrocera dorsalis
• Citrus tristeza virus
• Replicase and RNA mediated resistance
• Plant transformation
• RNA dependent RNA polymerase
• Coat protein genes
• Diploid wheat
• Particle bombardment
• Stable transformation
• Plant regeneration
• Transgene inheritance
• Agrobacterium mediated transformation
• Fluorescence reporter
• Transcript fusion
• BcZAT12 gene
• Zinc finger protein
• Transcription factor
• Drought salt tolerant
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• floral ontology
• embryogenesis
• micropropagation
• to functional genomics such as EST
• virus induced gene silencing
• Báo cáo lâm nghiệp hay
• báo cáo lâm nghiệp
• công trình nghiên cứu lâm nghiệp
• tài liệu về lâm nghiệp
• Protocolsfor Agrobacterium
• Direct transoformation
• biolistic process
• Concluding Remarks
• Binary vectors
• Turkish tobacco
• Protein expression
• Insect resistance
• Crop yield
• Osmotic treatment
• Cotton shoot regeneration
• Local cotton cultivar
• Cotton Gossypium hirsutum L