TAILIEUCHUNG - QTL mapping of some productivity and fibre traits in Gossypium arboreum

This study explains the construction of an intraspecific genetic linkage map of the A-genome diploid cotton with simple sequence repeat (SSR) and randomly amplified polymorphic DNA (RAPD) markers, using 180 F2 plants derived from the cross of 2 Gossypium arboreum cotton cultivars (Ravi × Entry-17). | Turkish Journal of Botany Turk J Bot (2013) 37: 802-810 © TÜBİTAK doi: Research Article QTL mapping of some productivity and fibre traits in Gossypium arboreum 1,2 1 1, Tayyaba SHAHEEN , Yusuf ZAFAR , Mehboob-ur RAHMAN * Plant Genomics and Molecular Breeding Laboratories, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan 2 Department of Bioinformatics and Biotechnology, GC University, Faisalabad, Pakistan 1 Received: Accepted: Published Online: Printed: Abstract: This study explains the construction of an intraspecific genetic linkage map of the A-genome diploid cotton with simple sequence repeat (SSR) and randomly amplified polymorphic DNA (RAPD) markers, using 180 F2 plants derived from the cross of 2 Gossypium arboreum cotton cultivars (Ravi × Entry-17). Polymorphisms between the 2 parents were detected using 1089 pairs of SSR primers and 520 RAPD primers. In total, 34 pairs of SSR primer-amplified and 18 RAPD primer-amplified polymorphic loci were selected to survey the F2 population. In total, 52 polymorphic DNA fragments were generated and used to construct a linkage map with JoinMap . A total of 45 loci and 5 phenotypic traits were mapped at a logarithm of odds ratio of ≥ on 10 linkage groups. The total length of the map was 346 cM, and the average distance between adjacent markers was cM. Chromosome assignments were made according to previous studies. In total, 7 quantitative trait loci (QTLs), including 5 for productivity traits and 2 for fibre traits, were mapped. Identification of these QTLs will set the stage for initiating marker-assisted selection and map-based cloning in cotton. Key words: Diploid cotton, Gossypium arboreum, simple sequence repeats, genetic map, quantitative trait loci, fibre, productivity traits 1. Introduction A-genome species of cotton including Gossypium arboreum L. and .

• Nông nghiệp
• Diploid cotton
• Gossypium arboreum
• Simple sequence repeats
• Genetic map
• Quantitative trait loci
• Productivity traits
• Cultivated tetraploid cotton species
• Ancestral diploid species
• Agenome species
• Cotton fibers
• Trichomes originating
• Plant Biology
• Diploid D genome cotton
• Co expression
• Comparative transcriptome
• Evolutionary divergence
• Shared network
• BMC Genomics
• Orthologous genes
• Duplicated pairs
• Divergent expression
• Long term evolution
• Gene diversity
• Polymorphic information content
• SSR markers
• Dolichos bean (Lablab purpureus L Sweet)
• Productivity per se traits
• Genetic diversity
• D2 statistics
• Intra and inter cluster distance
• Quality traits
• Genotypes for productivity
• Ginning outturn
• Line × Tester analysis
• Diverse lines
• Gossypium hirsutum L
• Fibre quality traits
• Hybrids derived
• Replacement of inorganic zinc
• Garden pea
• Morphophysiological traits
• Yield attributes
• Various methods
• Effect of integrated fertilization
• Integrated fertilization
• Integrated fertilization on qualitative
• Quantitative traits of Radish
• Maintaining sustainability in terms of productivity
• Principal component
• Mini core
• Sorghum [Sorghum bicolor (L
• ) Moench]
• Principal component analysis
• Grain quality
• Pearl millet
• Path analysis
• Parental lines
• Population hybrids
• Pearl millet (Pennisetum glaucum L R Br )
• Plant height
• Arachis hypogaea L
• Recombinant inbred line mapping population
• Genetic mapping in groundnut
• Parental polymorphism
• Natural variation
• Flowering time
• Genome wide association analysis
• Linkage analysis
• Gene expression
• Canola productivity