TAILIEUCHUNG - Epigallocatechin 3-gallate applications on HT-29 and MCF-7 cell lines and evaluation of tumor suppressor gene methylation
Epigallocatechin 3-gallate (EGCG) is an antitumor molecule and shows this activity by binding to the active center of a methyltransferase enzyme (DNMT1). The methylation of DNA sequences of tumor suppressor and DNA repair genes is observed in different stages of carcinogenesis. | Turkish Journal of Biology Turk J Biol (2015) 39: 698-704 © TÜBİTAK doi: Research Article Epigallocatechin 3-gallate applications on HT-29 and MCF-7 cell lines and evaluation of tumor suppressor gene methylation 1 1 2 1 1, Yunus Kasım TERZİ , Özge ÖZER KAYA , Özlem DARCANSOY İŞERİ , Zerrin ÇELİK , Feride İffet ŞAHİN * 1 Department of Medical Genetics, Faculty of Medicine, Başkent University, Ankara, Turkey 2 Institute of Transplantation and Gene Sciences, Başkent University, Ankara, Turkey Received: Accepted/Published Online: Printed: Abstract: Epigallocatechin 3-gallate (EGCG) is an antitumor molecule and shows this activity by binding to the active center of a methyltransferase enzyme (DNMT1). The methylation of DNA sequences of tumor suppressor and DNA repair genes is observed in different stages of carcinogenesis. In this study, we analyzed the effect of EGCG on the methylation status of 25 tumor suppressor genes in cancer cell lines HT-29 and MCF-7. HT-29 and MCF-7 cells were incubated with 10 µM, 20 µM, and 50 µM and 1 µM, 5 µM, and 10 µM EGCG for 48 h, respectively. We found promoter hypermethylation of (1) CDH13, GATA5, and RARβ genes in MCF-7 cell line and (2) RARβ, ESR1, PAX6, WT1, CADM1, CHFR, CDH13, and GATA5 genes in HT-29 cell line. However, (3) after EGCG application, no changes in methylation status were detected in our samples. Our results suggest that methylation status of tumor suppressor genes did not change with different EGCG doses. Key words: Epigallocatechin 3-gallate, epigenetics, methylation, breast cancer, colorectal cancer 1. Introduction EGCG is the major component of catechins found in green tea and exists in four active forms: (1)-epigallocatechin3-gallate (EGCG), (2)-epigallocatechin (EGC), (3)-epicatechin-3-gallate (ECG), and (4)-epicatechin (EC) (Zeng et al., 2014). Catechins, which are polyphenols, constitute nearly 30%–40% of
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