TAILIEUCHUNG - Ghrelin effects on midgut tissue antioxidative defense and glutathione S-transferase activity in Lymantria dispar (Lepidoptera)
The aim of the study was to examine changes in Cu‒Zn superoxide dismutase (SOD), catalase (CAT), their gel electrophoresis profiles, glutathione reductase (GR) activity, amount of glutathione (GSH), and the activity of glutathione S-transferase (GST) - phase II biotransformation enzyme in the midgut tissue of gypsy moth (Lymantria dispar L. (Lepidoptera: Lymantriidae)) larvae after ghrelin treatment. | Turkish Journal of Biology Research Article Turk J Biol (2015) 39: 618-623 © TÜBİTAK doi: Ghrelin effects on midgut tissue antioxidative defense and glutathione S-transferase activity in Lymantria dispar (Lepidoptera) Vesna PERIĆ-MATARUGA*, Milena VLAHOVIĆ, Marija MRDAKOVIĆ, Dragana MATIĆ, Anja GAVRILOVIĆ, Aleksandra MRKONJA, Larisa ILIJIN Department of Insect Physiology and Biochemistry, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia Received: Accepted/Published Online: Printed: Abstract: The aim of the study was to examine changes in Cu‒Zn superoxide dismutase (SOD), catalase (CAT), their gel electrophoresis profiles, glutathione reductase (GR) activity, amount of glutathione (GSH), and the activity of glutathione S-transferase (GST) - phase II biotransformation enzyme in the midgut tissue of gypsy moth (Lymantria dispar L. (Lepidoptera: Lymantriidae)) larvae after ghrelin treatment. Four subpicomolar injections of ghrelin ( pmol) or physiological saline (control) were applied every 24 h. The SOD, CAT, GR, GST activity, and amount of GSH were higher in the ghrelin-treated group than in the control. Electrophoresis gel bands of SOD and CAT had higher area and density in the treated group. The effects of ghrelin on the antioxidative defense and GST activity in insects were detected for the first time. The results provided evidence for possible application of insects as simple model systems in future studies of the role of ghrelin in the antioxidative protection of complex organisms. Key words: Superoxide dismutase, catalase, glutathione reductase, glutathione, glutathione S-transferase 1. Introduction Ghrelin, an appetite-stimulating hormone, is an important variable in the initiation of feeding behavior in invertebrates and vertebrates, including humans. Ghrelin, a 28-amino acid peptide, is an endogenous ligand for .
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