TAILIEUCHUNG - Apoptotic effect of cordycepin on A549 human lung cancer cell line
Cordycepin, an active ingredient in the insect fungus Cordyceps militaris, is in a category of compounds that exhibit significant therapeutic activity. The aim of the present study was to investigate the effect of cordycepin on cell proliferation, apoptosis, and cell cycle in A549 human lung cancer cells. | Turkish Journal of Biology Turk J Biol (2015) 39: 306-311 © TÜBİTAK doi: Research Article Apoptotic effect of cordycepin on A549 human lung cancer cell line 1, 2 3 1 Hardeep Singh TULI *, Gaurav KUMAR , Sardul Singh SANDHU , Anil Kumar SHARMA , Dharmbir KASHYAP 1 Department of Biotechnology, Maharishi Markandeshwar University, Mullana, Ambala, Haryana, India 2 Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana, India 3 Department of Biological Sciences, Rani Durgavati University, Jabalpur, Madhya Pradesh, India 4 Department of Histopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, Punjab, India Received: Accepted: Published Online: 4 Printed: Abstract: Cordycepin, an active ingredient in the insect fungus Cordyceps militaris, is in a category of compounds that exhibit significant therapeutic activity. The aim of the present study was to investigate the effect of cordycepin on cell proliferation, apoptosis, and cell cycle in A549 human lung cancer cells. MTT assay was used to evaluate the cytotoxic effect of cordycepin on A549 cell growth. Apoptotic effect was observed using cell morphology, DAPI staining, and DNA fragmentation studies. Flow cytometry (FCM) analysis was used to analyze cell cycle status after cordycepin treatment. Furthermore, apoptosis was assayed using annexin V-Alexa Fluor 488. Results of MTT assay showed that cordycepin significantly inhibited cell proliferation with an IC50 value of 64 µg/mL. The number of rounding-up cells increased with cordycepin treatment and changes in cellular morphology were seen. In DNA fragmentation studies, a typical ladder pattern was observed on agarose gel and formation of apoptotic bodies was further confirmed using DAPI staining. The FCM analysis of cordycepin-treated cells showed that apoptosis rate increased with the increase in dosage. In .
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