TAILIEUCHUNG - Differential expression of soluble pyrophosphatase isoforms in Arabidopsis upon external stimuli

In plants, pyrophosphate (PPi), generated in a wide range of reversible anabolic reactions, is hydrolyzed by pyrophosphatases. The presence of tonoplast- and Golgi-integral H+-translocating pyrophosphatases in plants led to the conclusion that plant cytosol has no soluble pyrophosphatase activity. | Turkish Journal of Botany Turk J Bot (2015) 39: 571-579 © TÜBİTAK doi: Research Article Differential expression of soluble pyrophosphatase isoforms in Arabidopsis upon external stimuli 1,2, 1 1 Zahide Neslihan ÖZTÜRK *, Steffen GREINER , Thomas RAUSCH Centre for Organismal Studies (COS), Plant Molecular Physiology, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany 2 Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technologies (FAST), Niğde University, Niğde, Turkey 1 Received: Accepted/Published Online: Printed: Abstract: In plants, pyrophosphate (PPi), generated in a wide range of reversible anabolic reactions, is hydrolyzed by pyrophosphatases. The presence of tonoplast- and Golgi-integral H+-translocating pyrophosphatases in plants led to the conclusion that plant cytosol has no soluble pyrophosphatase activity. However, the Arabidopsis thaliana (L.) Heynh. genome also encodes five soluble pyrophosphatase isoforms (PPas). There are almost no data in the literature on their redundancy in plant metabolism; therefore, we performed expression analyses of A. thaliana soluble pyrophosphatase isoforms in response to external stimuli including carbohydrate status, hormone action, and stress exposure. The results revealed pronounced specificity for each isoform. Interestingly, one isoform (PPa3; At2g46860) was specifically induced during seedling etiolation, and in the presence of the nonmetabolizable sugar 3-O-methylglucose. Based on quantitative PCR analyses, PPa1 (At1g01050) and PPa4 (At3g53620) appeared to be regulated by sugars. Quantitative PCR analyses indicated isoform- and tissue-dependent responses of PPa isoforms to ABA, salt, and cold stresses. PPa2 (At2g18230) and PPa5 (At4g01480) responded differentially to salinity, cold treatment, and ABA. We conclude that plant cytosolic pyrophosphatases perform multiple, so

• Nông nghiệp
• Cytosolic pyrophosphatase
• Arabidopsis thaliana L
• Heynh
• Salt stress
• Cold stress
• ABA response
• Phosphate starvation
• Carbohydrate regulation
• Cell biology of carbohydrate metabolism
• Regulation of metabolic pathways
• Glucose as a regulator of metabolism
• Metabolic control analysis
• Cell biology of carbohydrate
• Glucose as a regulator
• Plant Biology
• Lycoris radiata
• Propagation efficiency
• Signal transduction genes
• Carbohydrate metabolism
• Report medicine
• traditional medicine
• scientific reports
• biochemical studies
• environmental medicine
• Crystal structure
• Self regulation
• Self control
• Working memory
• Ego depletion
• Limited strength model of self control
• Learned industriousness
• Carbohydrate mouthwash
• Glucose swishing
• Carbohydrate polymers
• Water binding capacity
• Potato cell walls
• Rhamnogalacturonan I
• β galactosidase
• BMC Genomics
• Phlebia radiata
• Comparative genomics
• Wood decay
• Carbohydrate active enzyme genes
• Lignin biodegradation
• Co regulation
• Radix Astragali polysaccharide
• Microfold cell
• Lymphatic absorption
• Immune regulation