TAILIEUCHUNG - Whole-genome DNA methylation analysis in cotton (Gossypium hirsutum L.) under different salt stresses
Salt stress, one of the most important abiotic stresses, is a serious constraint on cotton production. Cytosine methylation in nuclear DNA, an epigenetic modification found in plants, animals, and other organisms, imparts an impressive wealth of heritable information upon the DNA code. | Turkish Journal of Biology Research Article Turk J Biol (2015) 39: 396-406 © TÜBİTAK doi: Whole-genome DNA methylation analysis in cotton (Gossypium hirsutum L.) under different salt stresses Xuke LU, Xiaojie ZHAO, Delong WANG, Zujun YIN, Junjuan WANG, Weili FAN, Shuai WANG, Tianbao ZHANG, Wuwei YE* State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, . China Received: Accepted/Published Online: Printed: Abstract: Salt stress, one of the most important abiotic stresses, is a serious constraint on cotton production. Cytosine methylation in nuclear DNA, an epigenetic modification found in plants, animals, and other organisms, imparts an impressive wealth of heritable information upon the DNA code. Although the cotton reference genome sequence is available to the public, the global DNA methylation data under different salinity stresses are still not available. Here, Zhong07 and ZhongS9612, salt-tolerant and salt-sensitive cultivars, respectively, were selected and methylation-sensitive amplification polymorphism (MSAP) technology was adopted to evaluate DNA methylation level alterations under different salt stresses in cotton. The findings indicated that different salt stresses exerted distinct effects on cotton seedling growth: specifically, both the neutral salt NaCl and alkalescent salt NaHCO3 showed relatively weak effects, while the alkaline salt Na2CO3 resulted in overt harm to seedlings, significantly darkening their caudexes and roots. MSAP analysis showed that after NaCl, NaHCO3, and Na2CO3 treatments, the DNA methylation levels of both leaves and roots decreased first before rising again. The trend in the roots for both type B (methylation) and C (demethylation) was identical to that observed in leaves; however, methylation levels had a different trend with the varying pH values of .
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