TAILIEUCHUNG - Differential DNA methylation under drought stress in maize

DNA methylation plays a vital roles in plant response to drought stress. Therefore, the present study was carried out to investigate differential cytosines methylation in leaves of maize under drought stress by using AMP method. Thirty days old maize seedlings were subjected to water deficit for nine days and the leaves of drought stressed plants and control plants were collected and stored at -20 until DNA extraction and subsequence DNA analysis. We isolated and sequenced 18 differentially methylated fragments. DMF 4, DMF 10, and DMF 13 were found to be homologous to lncRNA genes, DMF3 is similar to protein dehydrationinduced 19 homologue 3 and DMF 18 is homologous to nudix hydrolase 2 gene. The obtained results indicated that lncRNAs are consider as important regulators in response of maize plant to drought stress. | EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 08 2019 Journal homepage http Original Research Article https Differential DNA Methylation under Drought Stress in Maize Nehal Sallam1 Mounir Moussa1 Mohamed Yacout 1 and Ayman El-Seedy1 Department of Genetics Faculty of Agriculture Alexandria University Alexandria 21545 Egypt Corresponding author ABSTRACT Keywords DNA Methylation Drought Stress Maize DNA analysis Article Info Accepted 22 July 2019 Available Online 10 August 2019 DNA methylation plays a vital roles in plant response to drought stress. Therefore the present study was carried out to investigate differential cytosines methylation in leaves of maize under drought stress by using AMP method. Thirty days old maize seedlings were subjected to water deficit for nine days and the leaves of drought stressed plants and control plants were collected and stored at -20 until DNA extraction and subsequence DNA analysis. We isolated and sequenced 18 differentially methylated fragments. DMF 4 DMF 10 and DMF 13 were found to be homologous to lncRNA genes DMF3 is similar to protein dehydration-induced 19 homologue 3 and DMF 18 is homologous to nudix hydrolase 2 gene. The obtained results indicated that lncRNAs are consider as important regulators in response of maize plant to drought stress. Introduction Drought is one of the abiotic stresses that affect plant growth productivity and cause significant crop losses on annual basis. Therefore it is important to know how plants respond to drought stress by altering its biological activities. Plant faces abiotic stress by epigenetic changes that modify gene expression of the drought-responsive genes Setter et al. 2011 Boyer et al. 2012 Kakumanu et al. 2012 and Mao et al. 2015 . Epigenetic changes such as cytosine DNA methylation in plants play crucial roles in gene expression and silencing of transposon.

TỪ KHÓA LIÊN QUAN
TAILIEUCHUNG - Chia sẻ tài liệu không giới hạn
Địa chỉ : 444 Hoang Hoa Tham, Hanoi, Viet Nam
Website : tailieuchung.com
Email : tailieuchung20@gmail.com
Tailieuchung.com là thư viện tài liệu trực tuyến, nơi chia sẽ trao đổi hàng triệu tài liệu như luận văn đồ án, sách, giáo trình, đề thi.
Chúng tôi không chịu trách nhiệm liên quan đến các vấn đề bản quyền nội dung tài liệu được thành viên tự nguyện đăng tải lên, nếu phát hiện thấy tài liệu xấu hoặc tài liệu có bản quyền xin hãy email cho chúng tôi.
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.