TAILIEUCHUNG - The utilization of lignocellulosic wastes for laccase production under semisolid-state and submerged fermentation conditions

The aim of this study was to produce laccase enzymes by using various lignocellulosic wastes (LCWs) under semisolid-state (SsF) and submerged fermentation (SF) conditions. White rot fungi are the best laccase-producing organisms and they can be easily grown on LCW. | Turkish Journal of Biology Research Article Turk J Biol (2013) 37: 450-456 © TÜBİTAK doi: The utilization of lignocellulosic wastes for laccase production under semisolid-state and submerged fermentation conditions Emre BİRHANLI*, Özfer YEŞİLADA Department of Biology, Arts and Science Faculty, İnönü University, Malatya, Turkey Received: Accepted: Published Online: Printed: Abstract: The aim of this study was to produce laccase enzymes by using various lignocellulosic wastes (LCWs) under semisolid-state (SsF) and submerged fermentation (SF) conditions. White rot fungi are the best laccase-producing organisms and they can be easily grown on LCW. Thus, in this work, Trametes trogii (Berk.) ATCC 200800 and Trametes versicolor (L.) ATCC 200801, well-known laccase-producing white rot fungi, were used as laccase-producing organisms. According to the literature, some of the LCWs such as sunflower receptacle, apricot seed shell, and bulrush were tested first as substrates for laccase production by these fungi. In SsF, the maximum laccase activities were 2206 U/L for T. trogii incubated in a medium containing walnut shell and 387 U/L for T. versicolor incubated in a medium containing corncob. In SF, the highest laccase activities, 386 U/L and 1216 U/L, were obtained from T. trogii grown in a medium containing pulverized apricot seed shell and T. versicolor grown in a medium containing pulverized bulrush, respectively. Because laccase is an important biotechnological enzyme with widespread applications, it can be useful to utilize these natural substrates for laccase production by these fungi. Key words: Lignocellulosic waste, white rot fungi, fermentation, laccase, enzyme activity 1. Introduction The renewable material lignocellulose is the major component of biomass and consists of lignin, hemicellulose, and cellulose (1). It is the most abundant natural .

• Sinh học
• Lignocellulosic waste
• White rot fungi
• Enzyme activity
• Submerged fermentation
• Semisolid state
• Lignocellulosic biomass
• Second generation
• Future perspectives
• Second generation bioethanol production
• Lignocellulosics waste
• Millet straw
• Aspergillus flavus
• CMC agar medium
• Solid state fermentation
• Saccharomyces cerevisiae
• Cellulolytic yeasts
• Fruit wastes
• Production of ethano
• Wheat straw