TAILIEUCHUNG - Phenotypic and cytogenetic characterization of expanded adipose derived stem cells
In this study, three hASC samples from three Vietnamese people were collected and proliferated in MSCCult medium (Regemedlab, Ho Chi Minh City, Viet Nam) to the 5th cell passage. Next, hASCs were evaluated for change of mesenchymal stem cells (MSCs) characteristics including shape, immunophenotype (CD14, CD34, CD44, CD73, CD90, and/or CD166), and trilineage differentiation ability. Finally, the number of chromosomes after passages 1, 3, and 5 was evaluated by karyotyping technique. | Science and Technology Development Journal Vol 21, Issue 01, June 2018 Viet Nam National University Ho Chi Minh City, Viet Nam Science and Technology Development Journal ISSN: 1859-0128 Volume 21: Issue 01 June 2018 Journal homepage: Phenotypic and cytogenetic characterization of expanded adipose derived stem cells Nhat Chau Truong*, Anh Thi Van Vu, Vuong Minh Pham Laboratory of Stem Cell Research and Application, University of Science, Viet Nam National University, Ho Chi Minh City, Viet Nam. tcnhat@ ARTICLE DETAILS History Received: 18 May 2018 Accepted: 17 June 2018 Published: 21 June 2018 Keywords Adipose stem cells, Genomic integrity, Karyotype, Proliferation, Safety ABSTRACT Introduction: Human adipose derived stem cells (hASCs) have great potential for regenerative medicine. The demand for hASCs, especially in the development of off-the-shelf products, is increasing. Although the initial receipt of hASCs was relatively limited, there is now greater interest and also awareness that in vitro expansion of hASCs be further explored. The purpose of this study was to assess the integrity of mesenchymal cell characteristics and the mutant capability of chromosome number on hASCs undergoing in vitro : In this study, three hASC samples from three Vietnamese people were collected and proliferated in MSCCult medium (Regemedlab, Ho Chi Minh City, Viet Nam) to the 5th cell passage. Next, hASCs were evaluated for change of mesenchymal stem cells (MSCs) characteristics including shape, immunophenotype (CD14, CD34, CD44, CD73, CD90, and/or CD166), and trilineage differentiation ability. Finally, the number of chromosomes after passages 1, 3, and 5 was evaluated by karyotyping : The results showed that after five passages of culture, hASCs preserved the characteristic shape of MSCs, high expression of mesenchymal markers (. CD44, CD73, CD90, and CD166). However, the cells also .
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