TAILIEUCHUNG - Development of microsatellite markers in sesame (Sesamum indicum L.)

Sesame (Sesamum indicum L.) is an important oilseed crop with high-quality seed oil and many antioxidant properties. Owing to its commercial and medicinal values, there is a renewed interest among agricultural scientists in this ancient crop. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2014) 38: 603-614 © TÜBİTAK doi: Research Article Development of microsatellite markers in sesame (Sesamum indicum L.) 1, 2 2 2 2 Jyothi BADRI *, Vijay YEPURI , Anuradha GHANTA , Sivaramakrishnan SIVA , Ebrahimali Abubacker SIDDIQ 1 Directorate of Rice Research, Rajendranagar, Hyderabad, India 2 Institute of Biotechnology, Acharya N. G. Ranga Agricultural University, Rajendranagar, Hyderabad, India Received: Accepted: Published Online: Printed: Abstract: Sesame (Sesamum indicum L.) is an important oilseed crop with high-quality seed oil and many antioxidant properties. Owing to its commercial and medicinal values, there is a renewed interest among agricultural scientists in this ancient crop. Efforts to strengthen the sesame-specific marker base have been initiated in the recent past; however, the available number of microsatellite markers is still not sufficient for the development of high-resolution genetic linkage maps for important agronomic traits and there is a need to increase the number of informative DNA markers in sesame. In the present study, we developed 25 microsatellite markers by employing the selective hybridization strategy and 95 mining expressed sequence tags of the NCBI database. This new set of microsatellite markers was characterized and screened for genetic diversity in an array of 16 sesame germplasms. Of the 120 SSRs, 92 were polymorphic, consisting of 18 SSRs from selective hybridization and 74 from the EST data set. The number of alleles per microsatellite locus ranged from 2 to 5, with an average of alleles. The allele size ranged widely (100–510 bp) among the primer pairs. Polymorphic information content estimates ranged from to . Jaccard’s similarity coefficient ranged from to . The potential of the markers was assessed by .

• Nông nghiệp
• Microsatellite markers
• Antioxidant properties
• Medicinal values
• Agricultural scientists
• Ancient crop
• Kilakarsal sheep by Microsatellite markers
• Genetic diversity analysis
• Genetic variation detected by microsatellite markers
• Pigeonpea diversity
• Microsatellite (SSR) markers
• Genetic diversity assessment
• Pigeonpea cultivars
• Y chromosome analysis
• Y chromosomespecific microsatellite DNA markers
• Chloroform method
• Modern geographical locations
• Genetic diversity
• Evaluation of genetic variability
• Genetic variability in tomato
• Tomato (Solanum lycopersicum L
• Mill)
• Genotypes using microsatellite markers
• Labeo rohita
• River ganga
• Microsatellite DNA markers
• Conservation genetics
• Molecular markers
• Non invasive genotyping
• Conservation and management of wildlife
• Bread wheat
• Morphological characters
• Triticum aestivum
• Molecular characterization
• Genetic variability
• Structure of olive
• Olea europaea L
• Cross transferability
• DNA markers
• Expressed sequence tags
• Touch down PCR
• Expressed microsatellite
• Short tandem repeats
• SSR primer
• Similarity coefficient
• Cluster analysis
• Brassica juncea
• Drought tolerance in Indian mustard
• Brassica juncea L genotypes
• Prediction of suitable parents
• Analysis of allelic differentiation
• Simple sequence repeats
• Yellow rust
• Genic microsatellite markers
• Wheat genotypes
• Genetic characteristics in four farmed tilapia
• Genetic characteristics inpopulations evaluated by microsatellite markers
• Genetic characteristics inpopulations evaluated
• The genetic difference among populations
• Informative microsatellite markers
• Standard heterosis
• Coefficient of marker polymorphism
• Yield efficiency
• Heterosis predicibility
• Identification restorers
• Molecular marker
• Rice hybrids
• Restorer line
• Identification of rice hybrids
• New microsatellites
• Synonymous group
• Pomegranate accessions
• Hatchery and Dhaura reservoir
• Genetic variation
• Genetic variation in the natural populations
• Analysis of genetic variation
• Clarias batrachus
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Ampelopsis grossedentata
• Population structure
• Glycine max
• Cultivar identification
• Characterization of small ruminants
• Small ruminants
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• Báo cáo lâm nghiệp hay
• báo cáo lâm nghiệp
• công trình nghiên cứu lâm nghiệp
• tài liệu về lâm nghiệp
• Multiplex PCR
• PCR optimization
• Glycine max L
• SSR markers
• Multiplex microsatellite marker sets in soybean