TAILIEUCHUNG - E-microsatellite markers for some naturally occurring Salvia species in the Mediterranean region

The genus Salvia L. contains about 1000 species, more than 40 of which are endemic to the Mediterranean region. Species boundaries and classifications of this genus have been mainly conducted based on morphological and anatomical characteristics. | Turkish Journal of Biology Research Article Turk J Biol (2015) 39: 69-77 © TÜBİTAK doi: E-microsatellite markers for some naturally occurring Salvia species in the Mediterranean region Ayşe Gül İNCE, Mehmet KARACA* Department of Field Crops, Faculty of Agriculture, Akdeniz University, Antalya, Turkey Received: Accepted: Published Online: Printed: Abstract: The genus Salvia L. contains about 1000 species, more than 40 of which are endemic to the Mediterranean region. Species boundaries and classifications of this genus have been mainly conducted based on morphological and anatomical characteristics. Although genomic and genic microsatellites are commonly used in a variety of genetic studies in many plant and animal species, their use has been limited in Salvia species. This research was undertaken to study microsatellite contents of expressed sequence tags (ESTs) and develop a new set of expressed-microsatellite (e-microsatellite) markers using the GenBank databases. In in silico studies of the research, microsatellites were identified and e-microsatellite primer pairs flanking the repeats were designed. In in vitro studies of the present work, e-microsatellite primer pairs were used to amplify genomic DNA samples of different Salvia species naturally occurring in the Mediterranean region of Turkey. The results indicated that ESTs of related species deposited in GenBank were reliable sources for the development of e-microsatellite markers. The results of this study also indicated that cross-transferability of e-microsatellite markers decreased as the evolutionary distance among the tested Salvia species increased. E-microsatellite loci presented in this work could be very useful for measuring genetic diversity, gene flow, and species identification studies in the genus Salvia. Key words: Cross-transferability, DNA markers, expressed sequence tags, Labiatae,

• Sinh học
• Cross transferability
• DNA markers
• Expressed sequence tags
• Touch down PCR
• Expressed microsatellite
• Short tandem repeats
• BMC Genomics
• Functional relevance
• Hydrogen bond stability
• Hexa nucleotide repeats
• BMC Bioinformatics
• Whole genome sequencing
• DNA forensics
• Eukaryotic genomes
• BMC Genetics
• 3 dimensional models
• Protein structure
• Single nucleotide polymorphism
• Kinh population
• Y chromosome
• Extremely sensitive analysis method
• Gene diversity
• Mitochondrial DNA
• Precise annotation
• Short tandem repeat
• Next Generation Sequencing
• Protein coding genes
• Genome Biology
• DNA secondary structure
• Polymerase stalling
• Genome instability
• Genome evolution
• Non random
• Hierarchical clustering
• Significant biological implications
• Repeat expansions
• Whole genome sequencing data
• Genome wide analysis
• Friedreich ataxia
• Myotonic dystrophy type 1
• Atlantic cod
• Genome assembly
• NOD like receptors
• Gadus morhua
• Tri allelic pattern
• Chromosome 2
• X chromosome
• White tailed deer
• Odocoileus virginianus
• Panel evaluation