TAILIEUCHUNG - A presence of a substance binding with the specific antibody against domoic acid in the thorny oyster spondylus versicolor

This substance reacted well with DA-specific antibody in Western Blot experiment, but not corresponding to any standard protein. Our result indicates the occurrence of a certain non-protein substance, which was co-presented with DA in S. versicolor. Further investigation on chemical feature of this substance is under progress. | Journal of Marine Science and Technology; Vol. 14, No. 2; 2014: 149-154 ISSN: 1859-3097 A PRESENCE OF A SUBSTANCE BINDING WITH THE SPECIFIC ANTIBODY AGAINST DOMOIC ACID IN THE THORNY OYSTER SPONDYLUS VERSICOLOR Dao Viet Ha1,2 1 Institute of Oceanography, VAST School of Agriculture and Life Science, The University of Tokyo, Japan Email: daovietha69@ 2 Received: 13-3-2014 ABSTRACT: Domoic acid (DA) extracts from 10 specimens of a thorny oyster, Spondylus versicolor collected at Nha Phu bay, Khanh Hoa province, Vietnam in August, 2010 was analyzed individually by High Performance Liquid Chromatography (HPLC) and Indirect Enzyme Linked Immunosorbent Assay (ELISA). Domoic acid levels in the extracts detected by ELISA were always higher than those by HPLC, significantly. In the HPLC chromatogram, the peak of an unknown compound with retention time very close to DA, but different from the known DA isomers was always observed. This substance reacted well with DA-specific antibody in Western Blot experiment, but not corresponding to any standard protein. Our result indicates the occurrence of a certain non-protein substance, which was co-presented with DA in S. versicolor. Further investigation on chemical feature of this substance is under progress. Keywords: Spondylus versicolor, domoic acid, HPLC, ELISA, Vietnam INTRODUCTION Domoic acid (DA) was the marine toxin corresponding for Amnesic Shellfish Poisoning (ASP) case in Prince Edward Island, Canada in 1987 [1], and a microalgae Pseudonitzschia multiseries was identified as a causative organism[2]. Then, DA was known to be produced by at least 16 diatom species belonging to Pseudo-nitzschia genus [3-5] and Nitzschia navis-varingica [6]. After Canadian incident, no human poisoning corresponding to DA has been found, but this toxin has been found naturally in numerous planktivorous species [4]. DA is also often found to accumulate in organisms that are not filter feeding .

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