TAILIEUCHUNG - In vitro evaluation of PLLA/PBS sponges as a promising biodegradable scaffold for neural tissue engineering

In tissue engineering, the use of poly-L-lactic acid (PLLA)/polybutylene succinate (PBS) blend for the construction of scaffold is very limited. | Turkish Journal of Biology Turk J Biol (2017) 41: 734-745 © TÜBİTAK doi: Research Article In vitro evaluation of PLLA/PBS sponges as a promising biodegradable scaffold for neural tissue engineering 1,2 1,2,3 2,4 2,5, İrem Ayşe KANNECİ ALTINIŞIK , Fatma Neşe KÖK , Deniz YÜCEL , Gamze TORUN KÖSE * Molecular Biology-Genetics and Biotechnology Program (MOBGAM), İstanbul Technical University, Maslak, İstanbul, Turkey 2 Center of Excellence in Biomaterials and Tissue Engineering (BIOMATEN), Middle East Technical University, Ankara, Turkey 3 Department of Molecular Biology and Genetics, İstanbul Technical University, Maslak, İstanbul, Turkey 4 Department of Histology and Embryology, Faculty of Medicine, Acıbadem University, İstanbul, Turkey 5 Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, İstanbul, Turkey 1 Received: Accepted/Published Online: Final Version: Abstract: In tissue engineering, the use of poly-L-lactic acid (PLLA)/polybutylene succinate (PBS) blend for the construction of scaffold is very limited. Moreover, polymeric sponges fabricated from PLLA/PBS have not been studied for neural tissue engineering. In the present study, the potential of the utility of PLLA/PBS polymeric sponges seeded with Schwann cells was investigated. PLLA and PBS were blended in order to increase the processability and tune the crystallinity, porosity, and degradation rate of the resulted polymeric sponges. These sponges were then seeded with Schwann cells. Porosity analysis showed that there were no significant differences between different compositions of PLLA/PBS blends; however, the porosity was slightly higher in PLLA/PBS (3%, w/v, 2:1) scaffold. Degradation profiles were also investigated for 120 days and almost 25% weight of PLLA/PBS (6%, 4%, 2%, w/v, 1:1) scaffolds and 18% weight of PLLA/PBS (3%, w/v, 2:1) scaffolds were lost at the .

• Sinh học
• Neural tissue engineering
• Polybutylene succinate
• Poly L lactic acid
• Schwann cells
• Biologically active molecules