TAILIEUCHUNG - Extractions of Aspergillus flavus Link ex gray and Cladosporium cladosporioides (Fresen.) de vries from allergenic microfungi and application of toxicity tests

Aspergillus flavus Link ex Gray and Cladosporium cladosporioides (Fresen.) de Vries, isolated from the soil of Florya Atatürk Forest, were extracted and some toxicity tests were carried out in order to determine their toxic effects on mice for the first time in Turkey. | Turk J Biol 26 (2002) 33-36 © TÜB‹TAK Extractions of Aspergillus flavus Link ex Gray and Cladosporium cladosporioides (Fresen.) de Vries from Allergenic Microfungi and Application of Toxicity Tests Günay ÇOLAKO⁄LU Marmara University, Faculty of Arts and Sciences, Biology Department, 81040 Ziverbey, ‹stanbul -TURKEY e-mail: gtcolak@ Received: Abstract: Aspergillus flavus Link ex Gray and Cladosporium cladosporioides (Fresen.) de Vries, isolated from the soil of Florya Atatürk Forest, were extracted and some toxicity tests were carried out in order to determine their toxic effects on mice for the first time in Turkey. The method of extraction complied with the one described in the literature. During the process, Coca’s solution was used as an extractive agent. Sterile filtration was employed in the sterilization process. The stocks were diluted to 1/10 of their concentrations before the toxicity tests were carried out. It was found that the A. flavus Link ex Gray and C. cladosporioides (Fresen.) de Vries extracts were sterile and that the experiments performed on 10 mice proved to be not toxic. Key Words: Allergy, Aspergillus flavus and Cladosporium cladosporioides extracts, toxicity tests Allerjenik Mikrofunguslardan Aspergillus flavus Link ex Gray ve Cladosporium cladosporioides (Fresen.) de Vries’in Ekstrelerinin Haz›rlanmas› ve Toksisite Testi Uygulamalar› Özet: Aspergillus flavus Link ex Gray ve Cladosporium cladosporioides (Fresen.) de Vries Türkiye’de ilk defa Florya Atatürk Orman› topra¤›ndan izole edilmifl, ekstreleri haz›rlanm›fl ve toksik etkilerini tayin etmek için fareler üzerinde toksisite testi uygulamalar› yap›lm›flt›r. Ekstraksiyonlar literatürdeki metoda uygun olarak haz›rlanm›flt›r. Bu maksatla ekstraktif olarak Coca solüsyonu, sterilizasyon için steril filtrasyon tekni¤i kullan›lm›flt›r. Stok çözeltiler toksisite testi uygulamalar›ndan önce 1/10 oran›nda seyreltilmifltir. Haz›rlanan A. flavus Link ex Gray ve C. .

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