TAILIEUCHUNG - Impact of long term application of treated tannery effluents on the emergence of resistance traits in Rhizobium sp. isolated from Trifolium alexandrinum
A total of 35 Rhizobium sp. were isolated from the root nodules of Trifolium alexandrinum (Egyptian clover) irrigated with treated tannery effluents and characterised on the basis of morphological, cultural and biochemical characteristics. Rhizospheric soils and plant parts were also analysed for metal concentrations by atomic absorption spectrophotometry. | Turk J Biol 32 (2008) 1-8 © TÜB‹TAK Impact of Long-Term Application of Treated Tannery Effluents on the Emergence of Resistance Traits in Rhizobium sp. Isolated from Trifolium alexandrinum Mohd. Musheer ALTAF, Farhana MASOOD, Abdul MALIK Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202 002 - INDIA Received: Abstract: A total of 35 Rhizobium sp. were isolated from the root nodules of Trifolium alexandrinum (Egyptian clover) irrigated with treated tannery effluents and characterised on the basis of morphological, cultural and biochemical characteristics. Rhizospheric soils and plant parts were also analysed for metal concentrations by atomic absorption spectrophotometry. The test soil samples were contaminated with a high level of chromium and also with other heavy metals, . Ni, Zn, Cu, and Cd. The heavy metal analysis of Trifolium alexandrinum plant parts revealed different accumulation of these metals in different plant parts, such as root, stem, and leaf. Trifolium alexandrinum roots accumulated the highest amount of these metals and this was followed by leaves. All the 3+ 6+ 2+ 2+ 2+ 2+ isolates of Rhizobium sp. were tested for their resistance against Cr , Cr , Cd , Cu , Zn , and Ni . The highest minimum 3+ inhibitory concentration (MIC) of 1600 µg/ml was observed against Cr in of the isolates. Some of the metal resistant isolates that showed maximum resistance were also tested for their resistance against 6 commonly used antibiotics, namely tetracycline, ampicillin, gentamycin, kanamycin, chloramphenicol, and nalidixic acid. Forty percent of Rhizobium sp. isolates were resistant against nalidixic acid and were resistant to chloramphenicol and tetracycline. Key Words: Antibiotic resistance, metal resistance, soil, tannery effluents, Trifolium alexandrinum Trifolium alexandrinum’dan ‹zole Edilen Rhizobium sp’nin Dayan›kl›l›k Özelliklerinin Ortaya Ç›kmas›nda Dericilik .
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