TAILIEUCHUNG - Transmission of the embryonic stem cell (ESC) genome to offspring and sexual development in chimeric mice from a male Esc line

This study was performed to determine the capability of embryonic stem (ES) cells at late passage number to form functional germ cells and to orientate the sexual development of chimeric mice produced from ES cell injection into embryos. | Turk J Biol 24 (2000) 707–715 © TÜBİTAK Transmission of the Embryonic Stem Cell (ESC) Genome to Offspring and Sexual Development in Chimeric Mice From a Male Esc Line Sezen ARAT TÜBİTAK, MRC, Research Institute for Genetic Engineering and Biotechnology, Gebze, Kocaeli-TURKEY Received: Abstract: This study was performed to determine the capability of embryonic stem (ES) cells at late passage number to form functional germ cells and to orientate the sexual development of chimeric mice produced from ES cell injection into embryos. When an R1 ES cell line was routinely cultured on mitotically inactivated feeder cells in culture medium supplemented with leukemia inhibitory factor (LIF), the colonies maintained an undifferentiated ideal ES cell morphology. When the ES cells were injected into 45 eight-cell embryos, 38 embryos (84%) combined with ES cells. Twenty-eight percent of the embryos transferred to recipients developed to term. Thirty percent of offspring born were chimeric. In these chimeric individuals the contribution of ES cells to the coat was about 70% and 99% in two male and one female chimeras, respectively. Furthermore, ES cells contributed to the germ line in the female chimera whereas they did not contribute to the germ line in two male chimeras. This study showed that female chimeras may result from injected embryos with the male ES cell and may transmit the ES cell genome to their offspring. Key Words: Mouse, ES cells, chimera, sex conversion, germ cells. Bir Erkek Embriyonik Kök Hücre Hattından Elde Edilen Kimerik Farelerde Sexüel Gelişim ve Ek Hücrelerin Döle Aktarımı Özet: Bu çalışma embriyonik kök hücrelerin embriyoya enjeksiyonu ile üretilen kimerik farelerin sexüel gelişimini yönlendirmede ve fonksiyonal üreme hücrelerini üretmede geç pasaj sayısındaki embriyonik kök hücrelerin kabiliyetini belirlemek için yapıldı. R1 embriyonik kök (EK) hücre hattı, mitotik olarak inaktive edilmiş besleyici hücreler üzerinde ve lösemi inhibitör .

• Sinh học
• Embryonic stem cell
• Sex conversion
• Germ cells
• Functional germ cells
• Chimeric mice produced
• Embryonic stem cells
• Induced pluripotent stem cells
• Mesenchymal stem cells
• Stem cell based therapy
• Human embryonic stem cell
• Journal of Biology
• Cell cycle
• Cell death
• Endoderm differentiation
• Inner cell mass (ICM)
• Primordial germ cells
• Stem cells
• Genetic reprogramming
• Transcription factors
• Embryonic germ cells
• Genome Biology
• Single cell RNA seq
• Definitive endoderm
• Wave Crest
• Human pluripotent
• Stem cells into progenitor
• Human Embryonic
• Stem Cell
• Recruiting Donors
• Human embryonic stem cell N glycans
• N glycan core branching
• Human induced pluripotent stem cells
• Self renewal
• Glycan binding proteins
• BMC Molecular and Cell Biology
• Extracellular matrix
• Self renewal capacity
• Multiple stem cell markers
• Droplet digital PCR
• Cell culture
• Chromosome number
• Multiplex assay
• BMC Genomics
• Enhancer RNA
• Super enhancer
• Cell differentiation
• Stage specific seRNAs
• BMC Cancer
• Cell fusion
• Hepatocellular carcinoma
• Tumor initiating cell
• Human embryonic stem cells
• Sensory hair cell
• IPS cell
• Embryonic stem
• Induced pluripotent stem
• Parthenogenetic diploid
• Male embryo
• Embryo aggregation
• Blastulation rate
• Parthenogenetic embryonic stem cell isolation
• DNA methylation
• Cancer Epigenomics
• Cyclin dependent kinases
• Apoptotic stimuli
• Cell based therapeutic purposes
• Collagen type I
• Cyclic RGD peptide
• Confined compression
• Mechano transduction
• Regenerative medicine
• Cell reprogramming
• Ebook Chemical
• Functional genomic approaches
• Stem cell biology
• Adult stem cells
• Stem cells and regenerative medicine
• Nuclear reprogramming
• Stem cell fate
• Lineage commitment
• Transcription factor
• TF binding sequences
• Proximity ligation assay
• BMC Bioinformatics
• Stem cell pluripotency
• Non CpG methylation
• Pluripotent embryonic stem cells
• BMC Pediatrics
• Hematopoietic stem cells
• Very small embryonic like stem cells
• Peripheral blood circulating stem cells
• Blood testing
• Premature infants
• Alternative splicing
• Histone modification
• Cell fate decision
• Cell cycle machinery
• BMC Anesthesiology
• Human embryonic cell
• Heart failure
• Anesthetic preconditioning
• Cardiac progenitor cells
• Oxidative stress
• BMC Biotechnology
• Articular cartilage
• Osteoarthritis rats
• Embryonic body