TAILIEUCHUNG - Different fructose feeding strategies for poly(3 hydroxybutyrate) production byyangia sp. nd199
The bacterium could accumulate high PHB content during the exponential phase. Maximum cell dry weight (CDW) of g/l and PHB content of 49 wt% were obtained after 27 h of cultivation in batch fermentation. High CDW and PHB content were achieved by using fedbatch fermentation with different fructose feeding strategies. | Journal of Science and Technology 55 (2) (2017) 195-201 DOI: DIFFERENT FRUCTOSE FEEDING STRATEGIES FOR POLY(3-HYDROXYBUTYRATE) PRODUCTION BYYangia sp. ND199 Tran Huu Phong1, Dang Minh Khuong2, Duong Van Hop3, Doan Van Thuoc1, * 1 Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam 2 3 Bao Yen 2 High School, Bao Yen district, Lao Cai, Vietnam Institute of Microbiology and Biotechnology, 144 Xuan Thuy, Cau Giay, Hanoi, Vietnam *Email: thuocdv@ Received: 8 September 2016; Accepted for publication: 24 November 2016 ABSTRACT Yangia sp. ND199 is a halophilic bacterium isolated from mangrove soil sample. This strain was able to produce polyhydroxyalkanoate (PHA) from different carbon sources. Only homopolymer poly(3-hydroxybutyrate) (PHB) was synthesized when fructose was used as carbon source. The bacterium could accumulate high PHB content during the exponential phase. Maximum cell dry weight (CDW) of g/l and PHB content of 49 wt% were obtained after 27 h of cultivation in batch fermentation. High CDW and PHB content were achieved by using fedbatch fermentation with different fructose feeding strategies. The highest CDW of g/l, PHB content of wt%, and PHB productivity of 1 g/l/h were obtained by using two-stage fedbatch fermentation, is among the highest reported so far for PHB production by halophilic bacteria. Keywords: Yangia sp. ND199, fed batch fermentation, PHB production, fructose. 1. INTRODUCTION Plastic is a kind of material that is commonly used in everyday life in many forms. Most of plastics are made from petroleum oil, they are normally non-biodegradable materials. The use of petroleum-based plastics leads to big problem, because they accumulate in landfills and threaten the environment. The ecological drawbacks of petroleum-based plastics have pushed scientists to investigate and develop biodegradable plastics [1]. Polyhydroxyalkanoates (PHA) are polyester of .
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