TAILIEUCHUNG - Lecture Molecular biology (Fifth Edition): Chapter 4 - Robert F. Weaver

Chapter 4 review the fundamentals of gene structure and function, we are ready to start a more detailed study of molecular biology. The main focus of chapter will be the experiments that molecular biologists have performed to elucidate the structure and function of genes. | Molecular Biology Fifth Edition Chapter 4 Molecular Cloning Methods Lecture PowerPoint to accompany Robert F. Weaver Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Gene Cloning Gene cloning is an indispensable molecular biology technique that allows scientists to produce large quantities of their gene of interest Gene cloning links eukaryotic genes to small bacterial or phage DNAs and inserting these recombinant molecules into bacterial hosts Gene cloning can produce large quantities of these genes in pure form 4- The Role of Restriction Endonucleases Restriction endonucleases, first discovered in the late 1960s in E. coli, are named for preventing invasion by foreign DNA by cutting it into pieces These enzymes cut at sites within the foreign DNA instead of chewing from the ends By cutting DNA at specific sites they function as finely honed molecular knives 4- Naming Restriction Endonucleases Restriction endonucleases are named

• Sinh học
• Molecular biology
• Bài giảng Sinh học phân tử
• Sinh học phân tử
• Molecular cloning methods
• Gene cloning
• Directional cloning
• High Yield cell and molecular biology
• Cell and molecular biology
• The cell cycle
• Molecular biology of cancer
• Cell biology of the immune system
• Molecular biology techniques
• Identification of human disease genes
• Campbell biology
• Lecture Campbell biology
• Biological sciences
• Systems biology
• Molecular biology of the gene
• Chromosome replication
• Meiosis and genetic recombination
• The human nuclear genome
• The human mitochondrial genome
• Lecture Molecular biology
• Amino acids
• Molecular tools
• Studying genes
• History of genetics
• Both simple
• Molecular genetics
• Genetic code
• Molecular biology of microorganisms
• Different facets
• Plant molecular biology
• Genetic manipulation
• Recombinant DNA technology
• Molecular diagnostics
• Biochemistry cell and molecular biology test
• Molecular Biology Test
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• Structural Biology
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• Mendel’s Laws
• Transmission genetics
• Mendel’s gene transmission
• Genetic material
• Transforming material
• DNA structure
• Gene function
• Storing information
• Mechanism of transcription
• RNA polymerase structure
• Lac operon
• Bacterial transcription
• Sigma factor switching
• DNA Protein interactions
• The λ family of repressors
• Eukaryotic RNA polymerases
• RNA polymerases
• Class II factors
• Transcription activators in eukaryotes
• RNA polymerase II
• Histones in the nucleosome
• Nucleosome structure
• Messenger RNA processing I
• Genes in pieces
• RNA processing II
• Cap structure
• Cytoplasmic polyadenylation
• RNA processing
• Ribosomal RNA processing
• Transfer RNA processing