TAILIEUCHUNG - Lecture Molecular biology: Chapter 10 - Robert F. Weaver

Chapter 10 - Eukaryotic RNA polymerases and their promoters. In Chapter 6 we learned that bacteria have only one RNA polymerase, which makes all three of the familiar RNA types: mRNA, rRNA, and tRNA. In this chapter we will see that three distinct RNA polymerases occur in the nuclei of eukaryotic cells. Each of these is responsible for transcribing a separate set of genes, and each recognizes a different kind of promoter. | Molecular Biology Fourth Edition Chapter 10 Eukaryotic RNA Polymerases and Their Promoters Lecture PowerPoint to accompany Robert F. Weaver Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Multiple Forms of Eukaryotic RNA Polymerase There are at least two RNA polymerases operating in eukaryotic nuclei One transcribes major ribosomal RNA genes One or more to transcribe rest of nuclear genes Ribosomal genes are different from other nuclear genes Different base composition from other nuclear genes Unusually repetitive Found in different compartment, the nucleolus 10- Separation of the Three Nuclear Polymerases Eukaryotic nuclei contain three RNA polymerases These can be separated by ion-exchange chromatography RNA polymerase I found in nucleolus Location suggests in transcribes rRNA genes RNA polymerases II and III are found in the nucleoplasm 10- Roles of the Three RNA Polymerases Polymerase I makes large rRNA precursor Polymerase

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