TAILIEUCHUNG - Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis

The Rv0948c gene fromMycobacterium tuberculosisH37Rv encodes a 90 amino acid protein as the natural gene product with chorismate mutase (CM) activity. The protein, 90-MtCM, exhibits Michaelis–Menten kinetics with akcat of ± s )1 and a Kmof 1500 ± 100lmat 37 C and pH . The A˚ X-ray structure shows that 90-MtCM is an alla-helical homodimer (Protein Data Bank ID: 2QBV) with the topology ofEscheri-chia coliCM (EcCM), and that both protomers contribute to each catalytic site. Superimposition onto the structure of EcCM and the sequence align-ment shows that the C-terminus helix 3 is shortened | ễFEBS Journal A comparative biochemical and structural analysis of the intracellular chorismate mutase Rv0948c from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase y2828 from Yersinia pestis Sook-Kyung Kim Sathyavelu K. Reddyf Bryant C. Nelson Howard RobinsonỊ Prasad T. Reddy and Jane E. Ladner BiochemicalScience Division ChemicalScience and Technology Laboratory National institute of Standards and Technology Gaithersburg MD USA Keywords chorismate mutase Mycobacterium tuberculosis pathogenesis shikimate pathway Yersinia pestis Correspondence P. T. Reddy BiochemicalScience Division Bldg 227 Rm B244 ChemicalScience and Technology Laboratory National institute of Standards and Technology Gaithersburg MD 20899 USA Fax 1 301 975 8505 Tel 1 301 975 4871 E-mail J. E. Ladner BiochemicalScience Division Bldg 227 Rm B244 Chemical Science and Technology Laboratory National institute of Standards and Technology Gaithersburg MD 20899 USA Fax 1 240 314 6225 Tel 1 240 314 6206 E-mail Present addresses Division of Metrology for Quality Life Korea Research Institute of Standards and Science Daejeon Republic of Korea fDivision of Molecular Biology Department of Zoology Sri Venkateswara University Tirupati Andhra Pradesh India ỊBiology Department Brookhaven National Laboratory Upton NY USA Received 9 May 2008 revised 25 July 2008 accepted 30 July 2008 doi The Rv0948c gene from Mycobacterium tuberculosis H37Rv encodes a 90 amino acid protein as the natural gene product with chorismate mutase CM activity. The protein 90-MtCM exhibits Michaelis-Menten kinetics with a kcat of s 1 and a Km of 1500 100 pM at 37 C and pH . The A X-ray structure shows that 90-MtCM is an all a-helical homodimer Protein Data Bank ID 2QBV with the topology of Escherichia coli CM EcCM and that both protomers contribute to each catalytic site. Superimposition onto the structure of EcCM and the sequence

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