TAILIEUCHUNG - Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis

Glutathione reductase (GR) recycles oxidized glutathione (GSSG) by converting it to the reduced form (GSH) using an NADPH as the electron source. The function of GR in the male genital tract of the rat was examined by meas-uring its enzymatic activity and examining the gene expression and localization of the protein. | Eur. J. Biochem. 269 1570-1578 2002 FEBS 2002 The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis Tomoko Kaneko1 2 Yoshihito iuchi1. Takashi Kobavashi1 3 Tsuneko Fujii4 Hidekazu Saito2 w w 4 B B B w Bf B Bf B B B B B B Bf B w VBB B B B W B B B B B w w BB V BB B B B B B B B B w Bf B w B B B B B B BB B w BB w B F BB B Hirohisa Kurachi2 and Junichi Fujii1 Departments of1 Biochemistry 2Obstetrics and Gynecology and 3 Urology Yamagata University School of Medicine Yamagata Japan 4Cell Recovery Mechanisms RIKEN Brain Science Institute Japan Glutathione reductase GR recycles oxidized glutathione GSSG by converting it to the reduced form GSH using an NADPH as the electron source. The function of GR in the male genital tract of the rat was examined by measuring its enzymatic activity and examining the gene expression and localization of the protein. Levels of GR activity the protein and the corresponding mRNA were the highest in epididymis among testes vas deferens seminal vesicle and prostate gland. The localization of GR as evidenced by immunohistochemical techniques reveals that it exists at high levels in the epithelia of the genital tract. In testis GR is mainly localized in Sertoli cells. The enzymatic activity and protein expression of GR in primary cultured testicular cells confirmed its predominant expression in Sertoli cells. Intracellular GSH levels expressed as mol per mg protein was higher in sperma-togenic cells than in Sertoli cells. As a result of these findings the effects of buthionine sulfoximine BSO an inhibitor for GSH synthesis and 1 3-bis 2-chlorethyl -1- nitrosourea BCNU an inhibitor for GR on cultured testicular cells were examined. Sertoli cells were prone to die as the result of BCNU but not BSO treatment although intracellular levels of GSH declined more severely with BSO treatment. Spermatogenic cells were less sensitive to these agents than .

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