TAILIEUCHUNG - Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase

Bioremediation presents a potentially low cost and environmentally agreeable alternative to current physico-chemical remediation strategies. However, heavy metals such as mercury cannot be converted into non-toxic forms by naturally occurring bacteria. Annual global emissions estimates for mercury released into the environment are in the thousands of tons per year [1,2] while the remediation cost is in the thousands of dollars per pound. Finding new bioremediation technologies is an urgent need. Mercury is released into the environment as a result of human activities and natural events. Ionic and metallic forms of mercury can accumulate in sediments where they can be converted into highly toxic methyl mercury by bacteria. Further biomagnification of mercury through trophic levels. | Ruiz et al. BMC Biotechnology 2011 11 82 http 1472-6750 11 82 BMC Biotechnology RESEARCH ARTICLE Open Access Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase Oscar N Ruiz Derry Alvarez Gloriene Gonzalez-Ruiz and Cesar Torres Abstract Background The use of transgenic bacteria has been proposed as a suitable alternative for mercury remediation. Ideally mercury would be sequestered by metal-scavenging agents inside transgenic bacteria for subsequent retrieval. So far this approach has produced limited protection and accumulation. We report here the development of a transgenic system that effectively expresses metallothionein mt-1 and polyphosphate kinase ppk genes in bacteria in order to provide high mercury resistance and accumulation. Results In this study bacterial transformation with transcriptional and translational enhanced vectors designed for the expression of metallothionein and polyphosphate kinase provided high transgene transcript levels independent of the gene being expressed. Expression of polyphosphate kinase and metallothionein in transgenic bacteria provided high resistance to mercury up to 80 pM and 120 pM respectively. Here we show for the first time that metallothionein can be efficiently expressed in bacteria without being fused to a carrier protein to enhance mercury bioremediation. Cold vapor atomic absorption spectrometry analyzes revealed that the mt-1 transgenic bacteria accumulated up to pM of mercury from media containing 120 pM Hg. The extent of mercury remediation was such that the contaminated media remediated by the mt-1 transgenic bacteria supported the growth of untransformed bacteria. Cell aggregation precipitation and color changes were visually observed in mt-1 and ppk transgenic bacteria when these cells were grown in high mercury concentrations. Conclusion The transgenic bacterial system described in this study presents a viable

• Báo cáo khoa học
• bioremediation
• Mercury Resistance Bioassay
• Transgene Expres
• Mercury Conditions
• Total RNA
• Environmental pollution
• Pesticide residues
• Microbial bioremediation
• Microbial bioremediation of pesticide residues
• Bioremediation approach mostly
• E coli
• Bacterial species isolated
• Soil in bioremediation
• Bioremediation of diazinon
• Pirimicarb and atrazine pesticides
• Organophosphate pesticides
• In situ Bioremediation
• Bioremediation of chlorpyrifos
• Pesticide contaminated agricultural soil
• Agricultural soil
• BIOREMEDIATION OF AQUATIC
• TERRESTRIAL ECOSYSTEMS
• Modified Organisms
• Petroleum Contamination
• BTEX Hydrocarbons
• Reactive red
• Azo dye
• Strategic bioremediation of textile dye
• Bacterial decolorization
• Strategic bioremediation
• Determination of the efficiency
• Efficiency of the mixture
• Mixture of isolated bacterial species
• Bioremediation of pesticides
• Isolated bacterial species in bioremediation
• Mango orchard soil
• Pseudomonas mosselii
• Pseudomonas mosselii strain NG1
• Bacterial bioremediation of imidacloprid
• Bacterial bioremediation
• natural sciences
• science and technology
• scientific reports
• research projects
• scientific research
• Oil Recovery (EOR)
• Chemical EOR
• Fractured Reservoirs
• Through Bioremediation
• Microorganisms and Crude
• Metals Co Contamination
• Sinh vật chuyển gen
• Use of enzymes in bioremediation
• Ô nhiễm môi trường
• Phục hồi sinh học
• Phương pháp phục hồi sinh học
• Công nghệ sinh học
• Bacterial consortium
• Dehydrogenase activity
• Effective bioremediation
• Petroleum polluted saline soil
• Surfactant producing
• Pseudomonas aeruginosa consortium
• Study on bioremediation
• Growth curve patterns
• Bacteria cultured
• Hydrocarbon formulated media
• Different concentrations
• Petroleum hydrocarbon
• Plasmid DNA
• Bioremediation process
• Crude oil impacted tropical environment
• Heavy metals
• Rumen fluid
• Slaughter house
• Bioremediation of zinc
• Reduce heavy metals Zinc
• Ebook Mycoremediation
• Fungal bioremediation
• Fungal treatment
• Industrial wastewaters
• Fungal treatment of distillery
• Brewery wastes
• Fungal metabolism
• Polycyclic aromatic hydrocarbons
• Fungal lignin degradation
• Paper mill effluents
• science books
• wastewater treatment
• recalcitrant compounds
• contaminated soils
• Tạp chí Khoa học công nghệ và Thực phẩm
• Bioremediation of soil containing dioxins
• Bacteria in crude oil
• Soil containing dioxins
• Water phase in crude oil
• Dioxin in soil could decompose
• Bioremediation and detoxification
• Textile azo dye evans blue
• Bacterial strain AKIP 2
• Maximizing decolorization
• Physico chemical decomposition processes
• Acid orange
• Textile effluent
• Haloalkaliphilic bacterial consortium TVU
• Electron deficient xenobiotic chromogen
• Ecofriendly bioremediation
• Petrochemical waste
• Toluene toxicity
• Industrial effluent
• Microbial consortium obtained
• Bioremediation of toluene
• Azo dyes
• Bioremediation of textile
• Tannery effluents
• Human society
• General treatment methods
• môi trường sức khỏe
• ô nhiễm môi trường
• công nghiệp hóa học
• khoa học tự nhiên