TAILIEUCHUNG - Báo cáo khoa học: Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae

Structural analysis of the lipopolysaccharide (LPS) from nontypeableHaemophilus influenzaestrain 981 has been achieved using NMRspectroscopy and ESI-MS on O-deacylatedLPSand core oligosaccharide (OS)material as well as by ESI-MS n on permethylated dephosphorylated OS. A heterogeneous glycoform population was identified, resulting from the variable length of the OS branches attached to the glucose residue in the common structural element of H. influenzae LPS, L-a-D-Hepp-(1fi2)-[PEtnfi6]-L-a-D-Hepp-(1fi3)-[b-D-Glcp-(1fi4)]-L-a-D-Hepp-(1fi5)-[PPEtnfi4]-a-Kdop-(2fi6)-Lipid A | Eur. J. Biochem. 270 2979-2991 2003 FEBS 2003 doi Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae Martin Mansson1 Derek W. Hood2 E. Richard Moxon2 and Elke K. H. Schweda1 1 Clinical Research Centre Karolinska Institutet and University College of South Stockholm Novum Huddinge Sweden 2Molecular Infectious Diseases Group and Department of Paediatrics Weatherall Institute of Molecular Medicine John Radcliffe Hospital Oxford UK Structural analysis of the lipopolysaccharide LPS from nontypeable Haemophilus influenzae strain 981 has been achieved using NMR speclscscopy and ESI-MS on O-deacylated LPS and core oligosaccharide OS material as well as by ESI-MSn on permethylated dephosphorylated OS. A heterogeneous glycoform population was identified resulting from the variable length of the OS branches attached to the glucose residue in the common structural element of H. influenzae LPS L-a-D-Hepp- 1fi2 - PEtnfi6 -L-a-D-Hepp- 1fi3 - b-D-Glcp- 1fi4 -L-a-D-Hepp- 1fi5 - PPEtnfi4 -a-Kdop- 2fi6 -Lipid A. Notably the O-6 position of the b-D-Glcp residue was either substituted by PCho or the disaccharide branch b-D-Galp- 1fi4 -D-a-D-Hepp while the O-4 position was substituted by the globotetraose unit b-D-GalpNAc- 1fi3 -a-D-Galp- 1fi4 -b-D-Galp- 1fi4 -b-D-Glcp or sequentially truncated versions thereof. This is the first time a branching sugar residue has been reported in the outer-core region of H. influenzae LPS. Additionally a PEtn group was identified at O-3 of the distal heptose residue in the inner-core. Keywords Haemophilus lipopolysaccharide phosphocholine structural analysis ESI-MSn. Haemophilus influenzae is a Gram-negative pathogen that routinely colonizes the human upper respiratory tract and which can be found both in encapsulated types a-f and unencapsulated nontypeable forms. While the incidence of disease caused by H. influenzae type b invasive diseases .

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