TAILIEUCHUNG - Báo cáo khoa học: Transport of L-arginine and nitric oxide formation in human platelets

The results of the present study show that human platelets take up L-arginine by two transport systems which are compatible with the systems y + and y + L. These Na + -independent transporters have been distinguished by treat-ing platelets with N-ethylmaleimide that blocks selectively systemy + .Systemy + , that accounts for 30–40%of the total transport, is characterized by low affinity forL-arginine, is unaffected byL-leucine, is sensitive to changes of membrane potential and totrans-stimulation. . | Eur. J. Biochem. 270 2005-2012 2003 FEBS 2003 doi Transport of L-arginine and nitric oxide formation in human platelets Maria G. Signorello Raffaele Pascale and Giuliana Leoncini Dipartimento di Medicina Sperimentale sezione Biochimica Universitci di Genova Italy The results of the present study show that human platelets take up L-arginine by two transport systems which are compatible with the systems y and y L. These Na -independent transporters have been distinguished by treating platelets with N-ethylmaleimide that blocks selectively system y .Systemy that accounts for 30-40 of the total transport is characterized by low affinity for L-arginine is unaffected by L-leucine is sensitive to changes of membrane potential and to trans-stimulation. The other component of L-arginine transport identified with the system y L approximately 60-70 of the total flux shows high affinity for L-arginine is insensitive to N-ethylmaleimide treatment unaffected by changes in membrane potential sensitive to trans-stimulation and inhibited by L-leucine in the presence ofNa . Moreover a strict correlation between L-arginine transport and nitric oxide NO production in whole cells was found. N-ethylmaleimide and L-leucine decreased NO production as well as cGMP elevation and the effect on NO and cGMP were closely related. It is likely that the L-arginine transport systems y and y L are both involved in supplying substrate for NO production and regulation in human platelets. Keywords L-arginine nitric oxide platelets transport. The cationic amino acid L-arginine is the main source for the synthesis of nitric oxide NO in many cell types 1 . NO exerts different functions in the regulation of vascular tone and blood pressure and in neurotransmission in central nervous system 2 . One of the most relevant functions of NO is the inhibition of platelet aggregation 3 4 . The regulation of platelet activation is crucial to prevent platelet aggregation thrombus .

• Báo cáo khoa học
• scientific reports
• Report medicine
• traditional medicine
• Antimicrobial
• cell structure
• genetics
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• scientific research
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes