TAILIEUCHUNG - Báo cáo khoa học: Structure of the polysaccharide chain of the lipopolysaccharide from Flexibacter maritimus

Flexibacter maritimus, a Gram-negative bacterium, is a fish pathogenresponsible fordisease infinfishspecies andacause of cutaneous erosiondisease in sea-caged salmonids. For the development of serology based diagnostics, protective vac-cines, and a study of pathogenesis, the structural analysis of the lipopolysaccharide (LPS) producedby the bacteriumhas been undertaken. We now report that an acidic O-specific polysaccharide, obtained by mild acid degradation of the F. maritimusLPSwas found to be composed of a disac-. | Eur. J. Biochem. 270 1810-1815 2003 FEBS 3 doi 10J046 Structure of the polysaccharide chain of the lipopolysaccharide from Flexibacter maritimus Evgeny Vinogradov1 Leann L. MacLean1 Elizabeth M. Crump2 Malcolm B. Perry1 and William W. Kay2 Institute for Biological Sciences National Research Council Ottawa Ontario Canada Department of Biochemistry and Microbiology University of Victoria Victoria British Columbia Canada Flexibacter maritimus a Gram-negative bacterium is a fish pathogen responsible for disease in finfish species and a cause of cutaneous erosion disease in sea-caged salmonids. For the development of serology based diagnostics protective vaccines and a study of pathogenesis the structural analysis of the lipopolysaccharide LPS produced by the bacterium has been undertaken. We now report that an acidic O-specific polysaccharide obtained by mild acid degradation of the F. maritimus LPS was found to be composed of a disac- charide repeating unit built of 2-acetamido-3-O-acetyl-4- S -2-hydroxyglutar-5-ylamido -2 4 6-trideoxy-P-glucose and 5-acetamido-7- S -3-hydroxybutyramido -8-amino-3 5 7 8 9-pentadeoxynonulopyranosonic acid Sug having the structure The configuration of the C-2-C-7 fragment of the latter monosaccharide B was assigned b-manno however the configuration at C-8 could not be established. NMR data indicate that the two monosaccharides have opposite absolute configurations. The repeating unit includes a linkage via a S -2-hydroxyglutaric acid residue reported here for the first time as a component of a bacterial polysaccharide. The LPSwas also found to contain a minor amount of a disaccharide b-Sug- 2-3 -L-Rha isolated from the products of the acidic methanolysis of the LPS. Keywords Flexibacter maritimus lipopolysaccharide NMR polysaccharide. The Cytophaga - Flavobacterium - Flexibacter bacteria are a large somewhat heterogeneous group of filamentous gliding Gram-negative bacteria with unusual surface properties 1 . At

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