TAILIEUCHUNG - Báo cáo " Flavonoid glucosides from the leaves of Croton tonkinensis Gagnep., Euphorbiaceae "

Faculty of Chemistry, College of Natural Science, Vietnam National University Anticancer Agent Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea Summary Two flavone C-glucosides vitexin (1) and isovitexin (2) were isolated as the major constituents with the total content of of the methanol extract of the Croton tonkinensis leaves along with an minor acylated flavonol O-glucoside kaempferol 3-O- D-(6’’-O-coumaroyl)glucopyranoside (tiliroside, 3) () | Journal of Chemistry Vol. 42 1 P. 125 -128 2004 FLAVONOID GLUCOSIDES FROM THE LEAVES OF CROTON TONKINENSIS GAGNEP. EUPHORBIACEAE Received 14-7-2003 PHAN MINH GIANG1 JUNG JQQN LEE2 PHAN TONG SON1 Faculty of Chemistry College of Natural Science Vietnam National University 2Anticancer Agent Research Laboratory Korea Research Institute of Bioscience and Biotechnology Daejeon Korea SUMMARY Two flavone C-glucosides vitexin 1 and isovitexin 2 were isolated as the major constituents with the total content of of the methanol extract of the Croton tonkinensis leaves along with an minor acylated flavonol O-glucoside kaempferol 3-O-ft-D- 6 -O-coumaroyl glucopyr anoside tiliroside 3 . The structures of 1 2 and 3 were determined on the basis of ESIMS 1D and 2D NMR spectroscopic data. Although the flavonoid glucosides found exclusively in the ethyl acetate and n-butanol soluble fractions are reported for antioxidative and antiinflammatory activities their contribution to the medicinal properties of C. tonkinensis is demonstrated to be less than the lipid soluble ent- kaurene diterpenoid constituents of this plant In our past studies on the medicinal plant Croton tonkinensis Gagnep. Euphorbiaceae 1 the phytosterols the long chain alkyl alcohols and the ent-kaurene-type diterpenoids 2 were isolated from the non-polar parts n-hexane and CH2Cl2 soluble fractions of the methanol extract of the dried leaves of C. tonkinensis 3 . The investigation of the methanol extract by reserved-phase high performance liquid chromatography RP HPLC coupled with a photodiode array PDA detector 4 revealed the presence of two major classes of components ent-kaurene diterpenoids and flavonoid gluco-sides. Of the flavonoids two were quantified as the major total and one as the minor constituents of the methanol extract which were found to be localized in the polar ethyl acetate and n-butanol soluble fractions. When the ethyl acetate soluble fraction was dissolved in a minimum amount of .

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