TAILIEUCHUNG - Báo cáo khoa học: Mapping the functional domains of human transcobalamin using monoclonal antibodies

Recombinant human transcobalamin (TC) was probed with 17 monoclonal antibodies (mAbs), using surface plasmon resonance measurements. These experiments identified five distinct epitope clusters on the surface of holo-TC. Western blot analysis of the CNBr cleavage fragments of TC allowed us to distribute the epitopes between two regions, which spanned either the second quarter of the TC sequence GQLA TAAM(103–198) or the C-ter-minal peptide LEPA | ềFEBS Journal Mapping the functional domains of human transcobalamin using monoclonal antibodies - KI I- I 1 I 2 -I- 11 1-2 1 I -X I 3 I . I -I-1 d Sergey N. Fedosov1 Lars Orning2 Trond L0vli2 Edward V. Quadros3 Keith Thompson4 Lars Berglund5 and Torben E. Petersen1 1 Protein Chemistry Laboratory Department of Molecular Biology University of Aarhus Denmark 2 Axis-Shield AS Oslo Norway 3 Departments of Biochemistry and Medicine SUNY-Downstate MedicalCenter Brooklyn NY USA 4 Institute of Immunology Rikshospitalet University Hospital University of Oslo Norway 5 Cobento Biotech A S Aarhus Denmark Keywords antibodies cobalamin epitopes receptor transcobalamin Correspondence S. N. Fedosov Protein Chemistry Laboratory Department of Molecular Biology University of Aarhus Science Park Gustav Wieds Vej10 8000 Aarhus C Denmark Fax 45 86 13 65 97 Tel 45 89 42 50 92 E-mail snf@ Received 25 April 2005 revised 3 June 2005 accepted 6 June 2005 doi Recombinant human transcobalamin TC was probed with 17 monoclonal antibodies mAbs using surface plasmon resonance measurements. These experiments identified five distinct epitope clusters on the surface of holo-TC. Western blot analysis of the CNBr cleavage fragments of TC allowed us to distribute the epitopes between two regions which spanned either the second quarter of the TC sequence 103-198 or the C-ter-minal peptide 316-427 . Proteolytic fragments of TC and the synthetic peptides were used to further specify the epitope map and define the functional domains of TC. Only one antibody showed some interference with cobalamin Cbl binding to TC and the corresponding epitope was situated at the C-terminal stretch 372-399 . We explored the receptor-blocking effect of several mAbs and heparin to identify TC domains essential for the interaction between holo-TC and the receptor. The receptor-related epitopes were located within the TC sequence 103-159 . The .

• Báo cáo khoa học
• scientific reports
• Report medicine
• traditional medicine
• Structural biochemistry
• oxidation
• bacteria
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• scientific research
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes