TAILIEUCHUNG - Báo cáo khoa học: K1 - Basal Transcription Machinery

RNA polymerase II (pol II) produces all mRNA in eukaryotic cells. Structures of the ten-subunit core of yeast pol II in free form [1, 2], in complex with DNA and RNA [3], and in complex with alpha-amanitin [4] have elucidated the mRNA transcription mech-anism [reviewed in 5, 6]. Based on this work, models for the com-plete 12-subunit pol II were obtained [7, 8]. We have subsequently solved the first structure of pol II in complex with a transcription factor, the elongation factor TFIIS [9, 10]. | Abstracts K1 - Basal Transcription Machinery K1-001 Mechanism of the multiprotein RNA polymerase II P. Cramer Gene Center University of Munich Munich Germany. E-mail mewes@ RNA polymerase II pol II produces all mRNA in eukaryotic cells. Structures of the ten-subunit core of yeast pol II in free form 1 2 in complex with DNA and RNA 3 and in complex with alpha-amanitin 4 have elucidated the mRNA transcription mechanism reviewed in 5 6 . Based on this work models for the complete 12-subunit pol II were obtained 7 8 . We have subsequently solved the first structure of pol II in complex with a transcription factor the elongation factor TFIIS 9 10 . TFIIS stimulates a weak intrinsic RNA cleavage activity of pol II and is required for efficient mRNA proofreading and for escape from DNA arrest sites. A detailed model of this 13-polypeptide 536 kDa complex derived at A resolution shows a spectacular binding mode of TFIIS to the polymerase surface and reveals that pol II has a 465 Abstracts single tunable active site for both RNA polymerization and cleavage. Very recently we did complete the atomic structure of the 12-subunit pol II both in free form and in form of an elongation complex with bound DNA RNA and substrate NTP 11 12 . After a summary of this work I will present new structural and functional data that provide insights into the coupling of transcription to mRNA processing 13 and into pol II recycling 14 . References 1. Science 2000 288 640. 2. Science 2001 292 1863. 3. Science 2001 292 1876. 4. PNAS 2002 99 1218. 5. Curr Op Struc Biol 2002 12 89. 6. Bioessays 2002 24 724. 7. PNAS 2002 100 6964-6968. 8. PNAS 2003 100 6969-6972. 9. Cell 2003 114 347-357. 10. Curr Op Genet Dev 2004 in press . 11. JBC 2005 280 7131-7134. 12. Mol Cell 2004 16 955-965. 13. Nature 2004 430 223-226. 14. Mol Cell 2004 15 399-407. K1-002 The structure and function of Gfh GreA2 an inhibitor of RNA polymerase from Thermus thermophilus O. Laptenko1 J. Lee1 . Kim2 M.

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