TAILIEUCHUNG - Báo cáo khoa học: Mechanism of binding of serum response factor to serum response element

Serum response factor (SRF) is a MADS transcription factor that binds to the CArG box sequence of the serum response element (SRE). Through its binding to CArG sequences, SRF activates several muscle-specific genes as well as genes that respond to mitogens. The thermodynamic parameters of the interaction of core-SRF (the 124–245 fragment of serum response fac-tor) with specific oligonucleotides from c-fos anddesminpromoters, were determined by spectroscopy. | ềFEBS Journal Mechanism of binding of serum response factor to serum response element A I gyi o I 11 lot1 A ra Pa r I a Izi a n 1 a ri a-C I a I rn Ạ ma I H2 loo n-l l at io r lanHiòroe1 Piorro ala 3 HicA nuct Hid I ai laKiai I ividiicviaiic HII lauu Jcai Iiviaiic Jidi luicico I lene V aiat Serge Fermandjian4 Denise Paulin1 Bernard Alpert1 and Christian Zentz5 1 Biologie moleculaire de la differenciation Universite Paris 7 Paris France 2 Biocatalyse Bioregulation UMR 6204 CNRS Faculte des Sciences et des Techniques Universite de Nantes France 3 Materiaux Moleculaires UMR 7587 CNRS Thiais France 4 Biologie et Pharmacologie Structurales UMR 8113 CNRS Institut Gustave Roussy Villejuif and ENS 61 Cachan France 5 Biophysique Moleculaire Cellulaire Tissulaire UMR 7033 CNRS Universite Pierre et Marie Curie Site Serono-RN Evry France Keywords CArG-box desmin Fos fluorescence anisotropy MADS box Correspondence D. Paulin Biologie moleculaire de la differenciation Universite Paris 7 case 7136 2 place Jussieu 75251 Paris cedex 05 France Tel 33 1 44276994 Fax 33 1 44273615 E-mail paulin@ Received 28 February 2005 revised 8 April 2005 accepted 19 April 2005 doi Serum response factor SRF is a MADS transcription factor that binds to the CArG box sequence of the serum response element SRE . Through its binding to CArG sequences SRF activates several muscle-specific genes as well as genes that respond to mitogens. The thermodynamic parameters of the interaction of core-SRF the 124-245 fragment of serum response factor with specific oligonucleotides from c-fos and desmin promoters were determined by spectroscopy. The rotational correlation time of core-SRF labeled with bis-ANS showed that the protein is monomeric at low concentration 10 7 m . The titration curves for the fluorescence anisotropy of fluorescein-labeled oligonucleotide revealed that under equilibrium conditions the core-SRF monomers were bound sequentially to SRE at very .

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