TAILIEUCHUNG - Báo cáo khoa học: Oligosaccharide synthesis in Fibrobacter succinogenes S85 and its modulation by the substrate

In this article we compared the metabolism of phosphorylated and unphosphorylated oligosaccharides (cellodextrins and maltodextrins) in Fibrobacter succinogenes S85 resting cells incubated with the following substrates: glucose; cellobiose; a mixture of glucose and cellobiose; and cellulose. Intracellular and extracellular media were analysed by 1 H-NMR and by TLC. The first important finding is that no cellodextrins were found to accumulate in the extracellular media of cells, regardless of the substrate; this contrasts to what is generally reported in the literature | iFEBS Journal Oligosaccharide synthesis in Fibrobacter succinogenes S85 and its modulation by the substrate Regis Nouaille1 2 Maria Matulova1 3 Anne-Marie Delort1 and Evelyne Forano2 1 Laboratoire de Synthese et Etude de Systemes à Interet Biologique UMR 6504 Universite Blaise Pascal - CNRS 63177 Aubiere cedex France 2 Unite de Microbiologie INRA Centre de Recherches de Clermont-Ferrand-Theix 63122 Saint-Genes-Champanelle France 3 Institute of Chemistry Slovak Academy of Sciences Dubravska cesta 9 842 38 Bratislava Slovak Republik Keywords cellodextrin Fibrobacter succinogenes S85 maltodextrin NMR rumen Correspondence . Delort Laboratoire de Synthese et Etude de Systemes a Interet Biologique UMR 6504 Universite Blaise Pascal - CNRS 63177 Aubiere cedex France Fax 33 473 407717 Tel 33 473 407717 E-mail amdelort@ Received 15 December 2004 revised 1 March 2005 accepted 14 March 2005 doi In this article we compared the metabolism of phosphorylated and unphosphorylated oligosaccharides cellodextrins and maltodextrins in Fibrobacter succinogenes S85 resting cells incubated with the following substrates glucose cellobiose a mixture of glucose and cellobiose and cellulose. Intracellular and extracellular media were analysed by 1H-NMR and by TLC. The first important finding is that no cellodextrins were found to accumulate in the extracellular media of cells regardless of the substrate this contrasts to what is generally reported in the literature. The second finding of this work is that maltodextrins of degree of polymerization 2 are synthesized regardless of the substrate and can be used by the bacteria. Maltotriose plays a key role in this metabolism of maltodextrin. Maltodextrin-1-phosphate was detected in all the incubations and a new metabolite corresponding to a phosphorylated glucose derivative was produced in the extracellular medium when cells were incubated with cellulose. The accumulation of these .

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