TAILIEUCHUNG - Báo cáo khoa học: Planarian peptidylglycine-hydroxylating monooxygenase, a neuropeptide processing enzyme, colocalizes with cytochrome b561 along the central nervous system

Planarians are one of the simplest animal groups with a central nervous system. Their primitive central nervous system produces large quantities of a variety of neuropeptides, of which many are amidated at their C terminus. In vertebrates, peptide amidation is catalyzed by two enzymes [peptidylglycine a-hydroxylating monooxygenase (PHM) and peptidyl-a-hydroxylglycinea-amidating lyase] acting sequentially. In mammals, both enzymatic activities are contained within a single protein that is encoded by a single gene | iFEBS Journal Planarian peptidylglycine-hydroxylating monooxygenase a neuropeptide processing enzyme colocalizes with cytochrome b561 along the central nervous system Akikazu Asada1 Hidefumi Orii1 Kenji Watanabe1 and Motonari Tsubaki1 2 3 1 Department of Life Science Graduate Schoolof Life Science University of Hyogo formerly Himeji Institute of Technology Hyogo Japan 2 CREST Japan Science and Technology Agency JST Saitama Japan 3 Department of Molecular Science and MaterialEngineering Graduate Schoolof Science and Technology Kobe University Hyogo Japan Keywords peptidylglycine a-hydroxylating monooxygenase cytochrome b561 planarian neuroendocrine vesicle neuropeptide amidation Correspondence M. Tsubaki Department of Molecular Science and Material Engineering Graduate Schoolof Science and Technology Kobe University Rokkodai-cho Nada-ku Kobe Hyogo 657-8501 Japan Fax 81 78 803 6582 Tel 81 78 803 6582 E-mail mtsubaki@ Note The nucleotide sequence of planarian PHM in this article has been submitted to the DDBJ EMBL GenBank databases with accession number AB195502. Received 27 September 2004 revised 2 December 2004 accepted 14 December 2004 Planarians are one of the simplest animal groups with a central nervous system. Their primitive central nervous system produces large quantities of a variety of neuropeptides of which many are amidated at their C terminus. In vertebrates peptide amidation is catalyzed by two enzymes peptidylglycine a-hydroxylating monooxygenase PHM and peptidyl-a-hydroxylglycine a-amidating lyase acting sequentially. In mammals both enzymatic activities are contained within a single protein that is encoded by a single gene. By utilizing PCR with degenerate oligonucleotides derived from conserved regions of PHM we succeeded in cloning a full-length cDNA encoding planarian PHM. The deduced amino acid sequence showed full conservation of five His residues and one Met residue which bind two Cu atoms that are essential for the activity of PHM.

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