TAILIEUCHUNG - Báo cáo khoa học: Analysis of protein interactions within the cytokinin-signaling pathway of Arabidopsis thaliana

The signal of the plant hormone cytokinin is perceived by membrane-located sensor histidine kinases and transduced by other members of the plant two-component system. InArabidopsis thaliana, 28 two-component system proteins (phosphotransmitters and response regulators) act down-stream of three receptors, transmitting the signal from the membrane to the nucleus and modulating the cellular response. | ỊFEBS Journal Analysis of protein interactions within the cytokinin-signaling pathway of Arabidopsis thaliana Hakan Dortay1 Nijuscha Mehnert1 Lukas Burkle2 Thomas Schmulling1 and Alexander Heyl1 1 Institute of Biology Applied Genetics Free University of Berlin Germany 2 ETH Zurich Institute of Plant Sciences Zurich Switzerland Keywords cytokinin signal transduction two-component system yeast two-hybrid system protein-protein interaction Correspondence A. Heyl Institute of Biology Applied Genetics Free University of Berlin Albrecht-Thaer-Weg 6 D-14195 Berlin Germany Fax 49 30 838 54345 Tel 49 30 838 56550 E-mail heyl@ Received 6 June 2006 revised 25 July 2006 accepted 15 August 2006 doi The signal of the plant hormone cytokinin is perceived by membrane-located sensor histidine kinases and transduced by other members of the plant two-component system. In Arabidopsis thaliana 28 two-component system proteins phosphotransmitters and response regulators act downstream of three receptors transmitting the signal from the membrane to the nucleus and modulating the cellular response. Although the principal signaling mechanism has been elucidated redundancy in the system has made it difficult to understand which of the many components interact to control the downstream biological processes. Here we present a large-scale interaction study comprising most members of the Arabidopsis cytokinin signaling pathway. Using the yeast two-hybrid system we detected 42 new interactions of which more than 90 were confirmed by in vitro coaffinity purification. There are distinct patterns of interaction between protein families but only a few interactions between proteins of the same family. An interaction map of this signaling pathway shows the Arabidopsis histidine phosphotransfer proteins as hubs which interact with members from all other protein families mostly in a redundant fashion. Domain-mapping experiments revealed the interaction

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• breast cancer
• biological activities
• gastric cancer
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• cell proliferation
• chemical reaction
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine