TAILIEUCHUNG - CONFOCAL LASER MICROSCOPY - PRINCIPLES AND APPLICATIONS IN MEDICINE, BIOLOGY, AND THE FOOD SCIENCES

Science often grows exponentially from certain key insights, and the insight Marvin Minsky had in 1955 is no exception. While working as a postdoctoral fellow at HarvardUniversity, Minsky sought a method to reduce the high level of light scatter that made high contrast imag‐ ing of thick specimens impossible by standard light microscopy. He realized that an arrange‐ ment of lenses having conjugate focal planes (hence the term ‘confocal’), with a specimen and a pinhole placed at these opposite planes, could serve to reject the light that was scattered out- of-plane at the specimen. The result was a conceptually simple yet powerful method. | CONFOCAL LASER MICROSCOPY -PRINCIPLES AND APPLICATIONS IN MEDICINE BIOLOGY AND THE food sciences Edited by Neil Lagali Confocal Laser Microscopy - Principles and Applications in Medicine Biology and the Food Sciences http 50821 Edited by Neil Lagali Contributors Neil Lagali Beatrice Bourghardt Peebo Johan Germundsson Ulla Eden Reza Danyali Per Fagerholm Marcus Rinaldo Rita Marchi Emi Nishijima Sakanashi Herrera Marc Navarro Jun Fujita Natsuko Hemmi Shugo Tohyama Tomohisa Seki Yuuichi Tamura Keiichi Fukuda Akira Kobayashi Enzo Di Iorio Gary Chinga-Carrasco Magnus B. Lilledahl Catharina Davies M. Vitoria Bentley Anjali Basil Wahid Wassef Published by InTech Janeza Trdine 9 51000 Rijeka Croatia Copyright 2013 InTech All chapters are Open Access distributed under the Creative Commons Attribution license which allows users to download copy and build upon published articles even for commercial purposes as long as the author and publisher are properly credited which ensures maximum dissemination and a wider impact of our publications. After this work has been published by InTech authors have the right to republish it in whole or part in any publication of which they are the author and to make other personal use of the work. Any republication referencing or personal use of the work must explicitly identify the original source. Notice Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published chapters. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials instructions methods or ideas contained in the book. Publishing Process Manager Oliver Kurelic Technical Editor InTech DTP team Cover InTech Design team First published March 2013 Printed in Croatia A free online edition of this book is available at .

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