TAILIEUCHUNG - Characterization of ammonia oxidizing bacteria in an activated sludge plant using a fluorescent in situ hybridization method

The spatial arrangement of functionally important ammonia oxidizing bacteria (AOB) in activated sludge flocs is largely unknown and activated sludge flocs are regarded as heterogeneous agglomerations of bacterial communities. This study aimed to determine both the location of AOB microcolonies within flocs and the size variation of these microcolonies based on their location. | Turk J Biol 28 (2004) 1-7 © TÜB‹TAK Characterization of Ammonia Oxidizing Bacteria in an Activated Sludge Plant using a Fluorescent in situ Hybridization Method Gülnur COfiKUNER Department of Environmental Engineering, Faculty of Engineering, Cumhuriyet University, Sivas - TURKEY Received: Abstract: The spatial arrangement of functionally important ammonia oxidizing bacteria (AOB) in activated sludge flocs is largely unknown and activated sludge flocs are regarded as heterogeneous agglomerations of bacterial communities. This study aimed to determine both the location of AOB microcolonies within flocs and the size variation of these microcolonies based on their location. Fluorescent in situ hybridization (FISH) was used in combination with a confocal scanning laser microscope (CSLM) to achieve these goals. Grab samples taken from a full-scale plug flow wastewater treatment plant were examined with probes targeted to AOB βProteobacteria (Nso1225) and a genus specific probe targeted to the genus Nitrosospira (Nsv443). Frequencies of AOB microcolonies were observed to increase towards the center of activated sludge flocs (ASFs). In addition, the size of AOB microcolonies was found to rise from the edges to the centers of flocs. This study indicates that ASFs might have a non-random agglomeration of AOB microcolonies. However, FISH is a relatively preliminary method for the aims targeted in this study and the results are not sufficient to justify definite conclusions. Key Words: Activated Sludge Flocs, Ammonia Oxidizing Bacteria, Confocal Scanning Laser Microscopy, Fluorescent in Situ Hybridization Bir Aktif Çamur Sisteminde Amonya¤› Okside Eden Bakterilerin Yerinde Floresanl› Hibritleflme Yöntemi ile Tan›mlanmas› Özet: Amonya¤› okside eden bakterilerin (AOB) aktif çamur floklar› içerisindeki düzeni ço¤unlukla bilinmemektedir ve aktif çamur floklar› bakteri gruplar›n›n heterojen bir toplulu¤u olarak kabul edilmektedir. Bu çal›flmada, amonya¤› okside eden

Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.