TAILIEUCHUNG - In vitro study on the efficacy of silver nanoparticles against metallo-betalactamase and biofilm producing pseudomonas aeruginosa isolates

Metallo beta-lactamase (MBL) and biofilm production is the most worrisome resistant mechanisms observed in Pseudomonas aeruginosa. Increased resistance of Pseudomonas to antibiotic therapy is an emerging global health concern which can be overcomed by Silver nanoparticles (AgNPs). Therefore this study aims to evaluate the antibacterial and antibiofilm activity of Silver nanoparticles on MBL and biofilm producing P. aeruginosa isolates using Transmission Electron microscope (TEM) and Confocal laser scanning microscope (CLSM) respectively. A total 40 isolates of p. aeruginosa were screened for MBL production by combined disc test. Then the isolates were screened for biofilm forming ability by tissue culture plate assay. Commercially available Silver nanoparticles were obtained and tested against MBL and biofilm producing isolates. The antibacterial activity of AgNps was tested by MIC and MBC methods and by TEM. The CLSM results exhibited AgNPs concentration dependent inhibition of bacterial growth and EPS matrix of the biofilm. Therefore, AgNps serve as the next generation antimicrobials in protection against the biofilm mediated infections. It is suggested that coating of medical devices with silver nanoparticles is recommended for treatment of resistant bacterial infections. | In vitro study on the efficacy of silver nanoparticles against metallo-betalactamase and biofilm producing pseudomonas aeruginosa isolates

• Y khoa - Dược
• Silver nanoparticles
• Pseudomonas aeruginosa
• Metallo beta lactamase
• Transmission electron microscope
• Confocal laser scanning microscope
• Biosynthesis and characterization
• Characterization of silver nanoparticles
• Confirmed the synthesis of AgNPs
• Journal of Chemistry
• Fabrication of silver nanoparticles embedded polymer masterbatchs
• Excellent antibacterial performance
• Silver nanoparticles embedded polymer masterbatchs
• The silver nanoparticlesembedded polyethylene
• Gram negative Escherichia Coli
• Physiochemical properties
• Silver nanoparticles induced cytotoxicity
• Physicochemical properties
• Aggregation interaction
• Acinetobacter indicus
• Biomedical applications
• Biogenic synthesis
• Silver nanoparticles mediated
• Antimicrobial activity
• Pleurotus florida
• Their antimicrobial activity
• Silver nanoparticles from pleurotus florida
• Antibacterial activity
• Antibacterial activity of silver nanoparticles
• Antimicrobial agents could overcome mechanisms
• Quorum sensing
• Biosynthesised silver nanoparticles
• Achyranthes aspera
• Achyranthes aspera roots
• Maintaining different AgNO3 concentrations
• Bacillus sp DRI 6
• Biosynthesis of silver nanoparticles
• Medical sciences
• Citrus fruit peel extracts
• Synthesis of silver nanoparticles
• Different citrus fruit peel extracts
• Education research
• Antibacterial application against bacillus subtilis
• Chemical synthesis
• Phaseolus vulgaris L
• Copper nanoparticles
• In vivo
• Copper nanoparticles on physiological characteristics
• Trichoderma asperellum
• Physiological effects
• Camellia sinensis L
• Seed quality parameters
• Biosynthesized silver nanoparticles
• Gymnema sylvestre
• Antimicrobial activity (Fungi and Bacteria)
• Antimicrobial activities
• Green synthesis
• Silver fused azadirachta indica nanoparticles
• Producing staphylococcus aureus
• Green conjugates of silver nanoparticles
• Turkish Journal of Chemistry
• Tạp chí Hóa học
• Colorimetric detection
• Pharmaceutical analysis
• Polyvinyl alcohol citrate stabilized silver nanoparticles
• Anticancer activity
• Native silver resistant
• Subsequent antibacterial
• Silver nano
• Rhizoctonia solani
• Pseudomonas fluorescens
• Pathogen rhizoctonia solani kuhn
• Biogenic silver nanoparticles against rice
• Silver nanoparticle
• Phyllosphere associated bacterial strain
• Biogenic synthesis of silver nanoparticles
• BMC Chemistry
• Supported polysaccharide
• Silver sulfide
• Organic matter
• N–lauroylsarcosinate
• Lemongrass leaf
• Plant extract
• AgNO3 solution
• Al2O3 supported silver nanoparticles
• Thermal emission coefficient
• Physical method
• High antimicrobial activity
• Bonding capability
• Biogenic production
• Synthesis of metal nanoparticles
• Gram negative bacteria
• Geobacter sulfurreducens
• Nanoparticles synthesized
• Cinnamomum cassia
• Biological systems
• Sprague Dawley rats
• Surface enhanced raman scattering
• A layer of silver nanoparticles
• The metal nanoparticles synthesized
• Prepare SERS substrate