TAILIEUCHUNG - Báo cáo khoa học: Fifty years of muscle and the sliding filament hypothesis

This review describes the early beginnings of X-ray diffrac-tion work on muscle structure and the contraction mech-anismin the MRC Unit in the Cavendish Laboratory, Cambridge, and later work in the MRC Molecular Biology Laboratory in Hills Road, Cambridge, where the author worked for many years, and elsewhere. The work has depended heavily on instrumentation development, for which the MRC laboratory had made excellent provision. The search for ever higher X-ray intensity for time-resolved studies led to the development of synchrotron radiation as an exceptionally powerful X-ray source | Eur. J. Biochem. 271 1403-1415 2004 FEBS 2004 doi REVIEW ARTICLE Fifty years of muscle and the sliding filament hypothesis Hugh E. Huxley Rosenstiel Center Brandeis University Waltham MA USA This review describes the early beginnings of X-ray diffraction work on muscle structure and the contraction mechanism in the MRC Unit in the Ctiveddhh Lbooratory. Cambridge and later work in the MRC Molecular Biology Laboratory in Hills Road Cambridge where the author worked for many years and elsewhere. The work has depended heavily on instrumentation development for which the MRC laboratory had made excellent provision. The search for ever higher X-ray intensity for time-resolved studies led to the development of synchrotron radiation as an exceptionally powerful X-ray source. This led to the first direct evidence for cross-bridge tilting during force generation in muscle. Further improvements in technology have made it possible to study the fine structure of some of the X-ray reflections from contracting muscle during mechanical transients and these are currently providing remarkable insights into the detailed mechanism of force development by myosin cross-bridges. Keywords muscle structure contraction X-ray diffraction synchrotron radiation MRC Laboratory of Molecular Biology. Early days at the MRC 1948-1952 I came to the MRC Laboratory as a research student in the summer of 1948 when it was called the MRC Unit for Work on the Molecular Structure of Biological Systems and consisted of Max Perutz and John Kendrew who became my supervisor. Francis Crick joined the unit a short time later and Jim Watson was there during my last year as a graduate student. I had just finished Part II Physics in 1948 in my third year in Cambridge a degree interrupted by four years of working on radar development in the RAF during the war. Though extremely ignorant of biology I had picked up the idea that there might be interesting applications of physics to .

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