TAILIEUCHUNG - Báo cáo khoa học: Meiosis and small ubiquitin-related modifier (SUMO)conjugating enzyme, Ubc9

In this review, we describe the role of a small ubiquitin-like protein modi-fier (SUMO)-conjugating protein, Ubc9, in synaptonemal complex forma-tion during meiosis in a basidiomycete, Coprinus cinereus. Because its meiotic cell cycle is long and naturally synchronous, it is suitable for molecular biological, biochemical and genetic studies of meiotic prophase events. | IFEBS Journal REVIEW ARTICLE Meiosis and small ubiquitin-related modifier SUMO -conjugating enzyme Ubc9 Kengo Sakaguchi Akiyo Koshiyama and Kazuki Iwabata Department of Applied BiologicalScience Faculty of Science and Technology Tokyo University of Science Chiba Japan Keywords DNA polymerase Lim15 Dmc1 meiosis PCNA Rad51 SUMO sumoylation topoisomerase II Ubc9 Correspondence K. Sakaguchi Department of Applied Biological Science Faculty of Science and Technology Tokyo University of Science 2641 Yamazaki Noda-shi Chiba-ken 278 Japan Fax 81 471 23 9767 Tel 81 471 24 1501 ext. 3409 E-mail kengo@ Received 16 March 2007 revised 22 May 2007 accepted 30 May 2007 doi In this review we describe the role of a small ubiquitin-like protein modifier SUMO -conjugating protein Ubc9 in synaptonemal complex formation during meiosis in a basidiomycete Coprinus cinereus. Because its meiotic cell cycle is long and naturally synchronous it is suitable for molecular biological biochemical and genetic studies of meiotic prophase events. In yeast two-hybrid screening using the meiotic-specific cDNA library of C. cinereus we found that the meiotic RecA homolog CcLim15 interacted with CcUbc9 CcTopII and CcPCNA. Moreover both TopII and PCNA homologs were known as Ubc9 interactors and the targets of sumoylation. Immunocytochemistry demonstrates that CcUbc9 CcTopII and CcPCNA localize with CcLim15 in meiotic nuclei during leptotene to zygotene when synaptonemal complex is formed and when homologous chromosomes pair. We discuss the relationships between Lim15 Dmc1 CcLim15 TopII CcTopII PCNA CcPCNA and CcUbc9 and subsequently the role of sumoylation in the stages. We speculate that CcLim15 and CcTopII work in cohesion between homologous chromatins initially and then in the process of the zygotene events CcUbc9 works with factors including CcLim15 and CcTopII as an inhibitor of ubiquitin-mediated degradation and as a metabolic switch in the meiotic

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