TAILIEUCHUNG - Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin

The crystal structure ofPterocarpus angolensislectin is determined in its ligand-free state, in complex with the fucosylated biantennary complex type decasaccharide NA2F, and in complex with a series of smaller oligosaccha-ride constituents of NA2F. These results together with thermodynamic binding data indicate that the complete oligosaccharide binding site of the lectin consists of five subsites allowing the specific recognition of the penta-saccharide GlcNAcb(1–2)Mana(1–3)[GlcNAcb(1–2)Mana(1–6)]Man. . | ềFEBS Journal Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin Lieven Buts1 Abel Garcia-Pino1 Anne Imberty2 Nicolas Amiot3 Geert-Jan Boons3 Sonia Beeckmans4 Wim Versees1 Lode Wyns1 and Remy Loris1 1 Laboratorium voor Ultrastructuur Vrije Universiteit Brusseland Department of Molecular and Cellular Interactions Vlaams Interuniversitair Instituut voor Biotechnologie Belgium 2 Centre de Recherches sur les Macromolecules Vegetales CERMAV - CNRS affiliated with Joseph Fourier University Grenoble France 3 Complex Carbohydrate Research Center The University of Georgia Athens GA USA 4 Laboratorium voor Scheikunde der Protei nen Instituut voor Moleculaire Biologie Vrije Universiteit Brussel Belgium Keywords carbohydrate lectin legume lectin protein-carbohydrate recognition Correspondence R. Loris Laboratorium voor Ultrastructuur Vrije Universiteit Brusseland Department of Molecular and Cellular Interactions Vlaams Interuniversitair Instituut voor Biotechnologie Pleinlaan 2 B-1050 Brussels Belgium Fax 32 2 6291963 Tel 32 2 6291989 E-mail reloris@ The crystal structure of Pterocarpus angolensis lectin is determined in its ligand-free state in complex with the fucosylated biantennary complex type decasaccharide NA2F and in complex with a series of smaller oligosaccharide constituents of NA2F. These results together with thermodynamic binding data indicate that the complete oligosaccharide binding site of the lectin consists of five subsites allowing the specific recognition of the pentasaccharide GlcNAcP 1-2 Mana 1-3 GlcNAcP 1-2 Mana 1-6 Man. The mannose on the 1-6 arm occupies the monosaccharide binding site while the GlcNAc residue on this arm occupies a subsite that is almost identical to that of concanavalin A con A . The core mannose and the GlcNAcb 1-2 Man moiety on the 1-3 arm on the other hand occupy a series of subsites distinct from those of con A. Received 5 January 2006 revised 21 March 2006

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