TAILIEUCHUNG - Báo cáo khoa học: An intermediate step in the evolution of ATPases ) the F1F0-ATPase from Acetobacterium woodii contains F-type and V-type rotor subunits and is capable of ATP synthesis Michael Fritz and Volker Muller ¨

Previous preparations of the Na + F1F0-ATP synthase solubilized by Triton X-100 lacked some of the membrane-embedded motor subunits [Reidlinger J&Mu¨ller V (1994) Eur J Biochem233, 275–283]. To improve the subunit recovery, we revised our purification protocol. The ATP synthase was solu-bilized with dodecylmaltoside and further purified to apparent homogeneity by chromatographic techniques. | ỊFEBS Journal An intermediate step in the evolution of ATPases - the F1F0-ATPase from Acetobacterium woodii contains F-type and V-type rotor subunits and is capable of ATP synthesis Michael Fritz and Volker Muller Molecular Microbiology Bioenergetics Institute of Molecular Biosciences Johann Wolfgang Goethe University Frankfurt am Main Germany Keywords Acetobacterium woodii ATP synthase F-type rotor subunits V-type Correspondence V. Muller Molecular Microbiology Bioenergetics Institute of Molecular Biosciences Johann Wolfgang Goethe University Frankfurt Main Max-von-Laue-Str. 9 60438 Frankfurt Germany Fax 49 69 79829306 Tel 49 69 79829507 E-mail vmueller@ Received 23 March 2007 revised 2 May 2007 accepted 8 May 2007 Previous preparations of the Na F1F0-ATP synthase solubilized by Triton X-100 lacked some of the membrane-embedded motor subunits Reidlinger J Muller V 1994 Eur J Biochem 233 275-283 . To improve the subunit recovery we revised our purification protocol. The ATP synthase was solubilized with dodecylmaltoside and further purified to apparent homogeneity by chromatographic techniques. The preparation contained along with the F1 subunits the entire membrane-embedded motor with the stator subunits a and b and the heterooligomeric c ring which contained the V1V0-like subunit c1 and the F1F0-like subunits c2 and c3. After incorporation into liposomes ATP synthesis could be driven by an electrochemical sodium ion potential or a potassium ion diffusion potential but not by a sodium ion potential. This is the first demonstration that an ATPase with a V0-F0 hybrid motor is capable of ATP synthesis. doi Membrane-bound multisubunit ion-translocating ATP synthases ATPases are present in every domain of life. They arose from a common ancestor but evolved into three distinct classes of ATP synthases ATPases the F1F0-ATP synthase present in bacteria mitochondria and chloroplasts the A1A0-ATP synthase present in .

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