TAILIEUCHUNG - Báo cáo khoa học: Molecular imprinting of cyclodextrin glycosyltransferases from Paenibacillus sp. A11 and Bacillus macerans with c-cyclodextrin

Cyclodextrin glycosyltransferase catalyzes the formation of a mixture of cyclodextrins from starch by an intramolecular transglycosylation reaction. To manipulate the product specificity of thePaenibacillussp. A11 and Bacillus maceranscyclodextrin glycosyltransferases towards the preferential formation of c-cyclodextrin (CD8), crosslinked imprinted proteins of both cyclodextrin glycosyltransferases were prepared by applying enzyme imprinting and immobilization methodologies. | ỊFEBS Journal Molecular imprinting of cyclodextrin glycosyltransferases from Paenibacillus sp. A11 and Bacillus macerans with c-cyclodextrin Jarunee Kaulpiboon1 2 Piamsook Pongsawasdi3 and Wolfgang Zimmermann2 1 Department of Pre-ClinicalScience Biochemistry Faculty of Medicine Thammasat University Pathumthanee Thailand 2 Department of Microbiology and Bioprocess Technology Institute of Biochemistry University of Leipzig Germany 3 Starch and Cyclodextrin Research Unit Department of Biochemistry Faculty of Science Chulalongkorn University Bangkok Thailand Keywords cross-linked imprinted proteins cyclodextrin glycosyltransferase cyclodextrins molecular imprinting product specificity Correspondence W. Zimmermann Department of Microbiology and Bioprocess Technology Institute of Biochemistry University of Leipzig Leipzig 04103 Germany Fax 49 341 97 36798 Tel 49 341 97 36781 E-mail Website http . agz Received 3 November 2006 revised 11 December accepted 13 December 2006 doi Cyclodextrin glycosyltransferase catalyzes the formation of a mixture of cyclodextrins from starch by an intramolecular transglycosylation reaction. To manipulate the product specificity of the Paenibacillus sp. A11 and Bacillus macerans cyclodextrin glycosyltransferases towards the preferential formation of y-cyclodextrin CD8 crosslinked imprinted proteins of both cyclodextrin glycosyltransferases were prepared by applying enzyme imprinting and immobilization methodologies. The crosslinked imprinted cyclodextrin glycosyltransferases obtained by imprinting with CD8 showed pH and temperature optima similar to those of the native and immobilized cyclodextrin glycosyltransferases. However the pH and temperature stability of the immobilized and crosslinked imprinted cyclodextrin glycosyltransferases were higher than those of the native cyclodextrin glycosyltransferases. When the catalytic activities of the .

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