TAILIEUCHUNG - Báo cáo khoa học: Triose phosphate isomerase, a novel enzyme-crystallin, and s-crystallin in crocodile cornea High accumulation of both proteins during late embryonic development

Several enzymes are known to accumulate in the cornea in unusually high concentrations. Based on the analogy with lens crystallins, these enzymes are called corneal crystallins, which are diverse and species-specific. Exam-ining crystallins in lens and cornea in multiple species provides great insight into their evolution. | iFEBS Journal Triose phosphate isomerase a novel enzyme-crystallin and s-crystallin in crocodile cornea High accumulation of both proteins during late embryonic development Thandavarayan Kathiresan Kannan Krishnan Vaithilingam Krishnakumar Raman Agrawal Amit Anand Dasari Muralidhar Anurag K. Mishra Vishnu M. Dhople Ramesh K. Aggrawal and Yogendra Sharma Centre for Cellular and Molecular Biology UppalRoad Hyderabad-500 007 India Keywords cornea crocodile development T-crystallin a-enolase triose phosphate isomerase Correspondence Y. Sharma Centre for Cellular and Molecular Biology UppalRoad Hyderabad-500 007 India E-mail yogendra@ R. K. Aggrawal Centre for Cellular and Molecular Biology UppalRoad Hyderabad-500 007 India Fax 91 40 2716 0591 Tel 91 40 2716 0222 E-mail rameshka@ These authors contributed equally to this work Received 24 March 2006 revised 11 May 2006 accepted 26 May 2006 doi Several enzymes are known to accumulate in the cornea in unusually high concentrations. Based on the analogy with lens crystallins these enzymes are called corneal crystallins which are diverse and species-specific. Examining crystallins in lens and cornea in multiple species provides great insight into their evolution. We report data on major proteins present in the crocodile cornea an evolutionarily distant taxon. We demonstrate that T-crys-tallin a-enolase and triose phosphate isomerase TIM are among the major proteins expressed in the crocodile cornea as resolved by 2D gel electrophoresis and identified by MALDI-TOF. These proteins might be classified as putative corneal crystallins. T-Crystallin known to be present in turtle and crocodile lens has earlier been identified in chicken and bovine cornea whereas TIM has not been identified in the cornea of any species. Immunostaining showed that T-crystallin and TIM are concentrated largely in the corneal epithelium. Using western blot immunofluorescence and enzymatic activity

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