TAILIEUCHUNG - Báo cáo khoa học: Closely related colon cancer cell lines display different sensitivity to polyunsaturated fatty acids, accumulate different lipid classes and downregulate sterol regulatory element-binding protein 1

N-6 polyunsaturated fatty acids (PUFAs) may be associated with increased risk of colon cancer, whereas n-3 PUFAs may have a protective effect. We examined the effects of docosahexaenoic acid (DHA), eicosapentaenoic acid and arachidonic acid on the colon carcinoma cell lines SW480 derived from a primary tumour, and SW620 derived from a metastasis of the same tumour. | ềFEBS Journal Closely related colon cancer cell lines display different sensitivity to polyunsaturated fatty acids accumulate different lipid classes and downregulate sterol regulatory element-binding protein 1 Svanhild A. Sch0nberg1 Anne G. Lundemo1 Torill Fladvad1 Kristin Holmgren2 Hilde Bremseth2 Asbj0rn Nilsen2 Odrun Gederaas2 Kare E. Tvedt1 Kjartan W. Egeberg2 and Hans E. Krokan2 1 Department of Laboratory Medicine Children s and Women s Health Norwegian University of Science and Technology Trondheim Norway 2 Department of Cancer Research and Molecular Medicine Norwegian University of Science and Technology Trondheim Norway Keywords cell-cycle arrest lipid droplets lipid peroxidation polyunsaturated fatty acids SREBP1 Correspondence S. A. Schonberg Department of Laboratory Medicine Children s and Women s Health Norwegian University of Science and Technology Laboratoriesenteret St. Olav s Hospital N-7006 Trondheim Norway Fax 47 725 76400 Tel 47 725 73362 E-mail Received 10 March 2006 accepted 24 April 2006 doi N-6 polyunsaturated fatty acids PUFAs may be associated with increased risk of colon cancer whereas n-3 PUFAs may have a protective effect. We examined the effects of docosahexaenoic acid DHA eicosapentaenoic acid and arachidonic acid on the colon carcinoma cell lines SW480 derived from a primary tumour and SW620 derived from a metastasis of the same tumour. DHA had the strongest growth-inhibitory effect on both cell lines. SW620 was relatively more growth-inhibited than SW480 but SW620 also had the highest growth rate in the absence of PUFAs. Flow cytometry revealed an increase in the fraction of cells in the G2 M phase of the cell cycle particularly for SW620 cells. Growth inhibition was apparently not caused by increased lipid peroxidation reduced glutathione or low activity of glutathione peroxidase. Transmission electron microscopy revealed formation of cytoplasmic lipid droplets after DHA .

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