TAILIEUCHUNG - Báo cáo khoa học: Molecular mechanisms regulating molting in a crustacean Halagowder Devaraj and Ayithan Natarajan

Crustacean growth and development is characterized by periodic shedding (ecdysis) and replacement of the rigid exoskeleton. Secretions of the X-organ sinus gland complex control the cellular events that lead to growth and molting. Western blot and ELISA results showed a progressive increase in growth arrest-specific protein (Gas7) from early postmolt stage to a maximum at late postmolt stage. | ềFEBS Journal Molecular mechanisms regulating molting in a crustacean Halagowder Devaraj and Ayithan Natarajan Unit of Biochemistry Department of Zoology University of Madras TamilNadu India Keywords crustaceans ecdysteroid growth arrestspecific protein Gas7 molt-inhibiting hormone X-organ sinus gland complex Correspondence H. Devaraj Unit of Biochemistry Department of Zoology University of Madras Life Sciences Building Guindy Campus Chennai 600 025 TamilNadu India Fax 91 44 22301003 Tel 91 44 22200574 E-mail hdrajum@ Received 20 September 2005 revised 15 December 2005 accepted 22 December 2005 doi Crustacean growth and development is characterized by periodic shedding ecdysis and replacement of the rigid exoskeleton. Secretions of the X-organ sinus gland complex control the cellular events that lead to growth and molting. Western blot and ELISA results showed a progressive increase in growth arrest-specific protein Gas7 from early postmolt stage to a maximum at late postmolt stage. Phosphorylation of ERK2 a downstream signaling protein was also identified in the subsequent stages. ERK2 phosphorylation resulted in the expression of molt-inhibiting hormone MIH . Specific ERK inhibitors PD98059 and UO126 exhibited the ability to reduce the molting duration of Fenneropenaeus indicus from 12-14 days to 7-8 days suggesting that the ERK1 2 signaling pathway is responsible for the expression of MIH which controls the molt cycle. We have identified the stage-specific expression of Gas7 w 48 kDa in the X-organ sinus gland complex of eyestalk which is involved in the downstream signaling of the ERK1 2 pathway regulating the expression of MIH during the molt cycle of the white shrimp F. indicus. These are the first data showing an association between the Gas7 signal-transduction process and regulation of the molt cycle and provides an alternative molecular intervention mechanism to the traditional eyestalk ablation in crustaceans. .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• intermediate cells
• cell proliferation
• medical analysis
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine